CD133 Antibody (PROM/6316) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08969

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PROM/6316

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD133 Antibodies »

Product Specifications

Immunogen

Recombinant chimericCD133 protein (around aa180-380 and aa612-765). This Mab recognizes both glycosylated and non-glycosylated extracellular domains of CD133. (Uniprot: O43490)

Localization

Apical cell Membrane Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment

Marker

Cancer Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07898).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for CD133 Antibody (PROM/6316) - Azide and BSA Free

Immunohistochemistry-Paraffin: CD133 Antibody (PROM/6316) - Azide and BSA Free [NBP3-08969]

Immunohistochemistry-Paraffin: CD133 Antibody (PROM/6316) - Azide and BSA Free [NBP3-08969]

Immunohistochemistry-Paraffin: CD133 Antibody (PROM/6316) - Azide and BSA Free [NBP3-08969] - Formalin-fixed, paraffin-embedded human papillary renal cell carcinoma stained with CD133 antibody (PROM/6316) - Azide and BSA Free.
Immunohistochemistry-Paraffin: CD133 Antibody (PROM/6316) - Azide and BSA Free [NBP3-08969]

Immunohistochemistry-Paraffin: CD133 Antibody (PROM/6316) - Azide and BSA Free [NBP3-08969]

Immunohistochemistry-Paraffin: CD133 Antibody (PROM/6316) - Azide and BSA Free [NBP3-08969] - Formalin-fixed, paraffin-embedded human papillary renal cell carcinoma stained with CD133 antibody (PROM/6316) - Azide and BSA Free.

Applications for CD133 Antibody (PROM/6316) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD133

Prominin 1 (CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The CD133 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Alternate Names

AC133, CD133, PROM1, Prominin 1, PROML1

Gene Symbol

PROM1

Additional CD133 Products

Product Documents for CD133 Antibody (PROM/6316) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD133 Antibody (PROM/6316) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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