CEACAM1/CD66a Antibody (CC1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43390
![Immunoprecipitation: CEACAM1/CD66a Antibody (CC1) - BSA Free [NBP1-43390]](https://resources.bio-techne.com/images/products/CEACAM1-CD66a-Antibody-CC1-Immunoprecipitation-NBP1-43390-img0001.jpg "Immunoprecipitation: CEACAM1/CD66a Antibody (CC1) - BSA Free [NBP1-43390]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, In vitro assay, Western Blot
Cited:
Block/Neutralize, Flow Cytometry, Immunohistochemistry-Frozen, Immunoprecipitation, In vitro assay, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CC1
Format
BSA Free
Concentration
5 mg/ml
Product Specifications
Immunogen
The immunogen for this antibody was CD66a.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CEACAM1/CD66a Antibody (CC1) - BSA Free
Immunoprecipitation: CEACAM1/CD66a Antibody (CC1) - BSA Free [NBP1-43390]
Immunoprecipitation: CEACAM1/CD66a Antibody (CC1) [NBP1-43390] - CEACAM1 interacts with Syk. Immunoblot analysis of CEACAM1, Syk, and GAPDH in mouse B-lymphocyte from whole cell lysate and after immunoprecipitation with or without anti-CEACAM1 antibody (mAb, CC1). Data are representative of 3 independent experiments. Image collected and cropped by CiteAb from the following publication (nature.com/articles/ncomms7217) licensed under a CC-BY license.Applications for CEACAM1/CD66a Antibody (CC1) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Application Notes
This CC1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in Immunohistochemistry-Frozen, Immunoprecipitation, western blot, In vitro assay, blocking / neutralizing, and FLOW reported in scientific literature (PMID 25692415). Use in ICC/IF was reported in scientific literature (PMID: 25692415).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CEACAM-1/CD66a
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 1
Alternate Names
BGP1, Biliary Glycoprotein 1, CD66a, Cea-1, CEACAM1, Hv-1, Hv-2, MHVR
Entrez Gene IDs
1048 (Human)
Gene Symbol
CEACAM5
UniProt
Additional CEACAM-1/CD66a Products
Product Documents for CEACAM1/CD66a Antibody (CC1) - BSA Free
Product Specific Notices for CEACAM1/CD66a Antibody (CC1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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