Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Knockout Validated, Western Blot

Cited:

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CHD4 3F2/4

Format

BSA Free

Concentration

1 mg/ml

View all CHD4 Antibodies »

Product Specifications

Immunogen

Synthetic peptide (N- and C- terminal peptides were used for immunisation. 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ. Which peptide the antibody recognises has not been tested)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for CHD4 Antibody (CHD4 3F2/4) - BSA Free

Flow Cytometry: CHD4 Antibody (CHD4 3F2/4) - BSA Free [NBP2-50163]

Flow Cytometry: CHD4 Antibody (CHD4 3F2/4) - BSA Free [NBP2-50163]

Flow Cytometry: CHD4 Antibody (CHD4 3F2/4) [NBP2-50163] - An intracellular stain was performed on HeLa cells with CHD4 Antibody (CHD4 3F2/4) NBP2-50163PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Applications for CHD4 Antibody (CHD4 3F2/4) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
This antibody is Cytof ready. Use in Knockout Validated reported in scientific literature (PMID: 31042474).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHD4

CHD4/Mi2beta is a helicase component of the nucleosome remodeling and deacetylase (NuRD) complex that functions to remodel chromatin and repress transcription. Outside of the NuRD complex, CHD4/Mi2beta can function as an activator of transcription in association with p300 histone acetyltransferase. Alternate names for CHD4/Mi2beta include chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, Mi-2b, and DKFZp686E06161.

Alternate Names

ATP-dependent helicase CHD4, CHD-4, chromodomain helicase DNA binding protein 4, chromodomain-helicase-DNA-binding protein 4, DKFZp686E06161, EC 3.6.1, EC 3.6.4.12, Mi-2 autoantigen 218 kDa protein, Mi-2b, Mi2-beta

Gene Symbol

CHD4

Additional CHD4 Products

Product Documents for CHD4 Antibody (CHD4 3F2/4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CHD4 Antibody (CHD4 3F2/4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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