Chromogranin A Antibody (SPM553)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32955

Novus Biologicals, part of Bio-Techne
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NBP2-32955-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM553

Concentration

0.2 mg/ml

View all Chromogranin A Antibodies »

Product Summary for Chromogranin A Antibody (SPM553)

Immunogen

Recombinant human chromogranin A protein (Uniprot: P10645)

Localization

Finely granular cytoplasmic

Marker

Neuroendocrine Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34795)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Chromogranin A Antibody (SPM553)

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody (SPM553) [NBP2-32955]

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody (SPM553) [NBP2-32955]

Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody (SPM553) [NBP2-32955] - SHSY5Y cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Chromogranin A conjugated to Alexa Fluor 488 [NBP2-34795AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the Alexa Fluor 488 version of this antibody.
Immunohistochemistry-Paraffin: Chromogranin A Antibody (SPM553) [NBP2-32955]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (SPM553) [NBP2-32955]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (SPM553) [NBP2-32955] - Formalin-fixed, paraffin-embedded human pancreas stained with chromograninn A Monoclonal Antibody (SPM553)

Applications for Chromogranin A Antibody (SPM553)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Chromogranin A

Chromogranin A (CgA) is an 86 kDa protein that is the major member of the granin family of acidic secretory glycoproteins located in neuroendocrine cells. CgA is believed to play a role in targeting peptide hormones and neurotransmitters to granules of the regulated pathways and inhibit hormone and neurotransmitter release. Also, the widespread distribution of CgA has made the measurement of circulating immunoreactive CgA a valuable tool in the diagnosis of neuroendocrine neoplasia, and CgA immunohistochemistry can help to identify the neuroendocrine nature of tumors. The N-terminal domain of CgA inhibits tumor necrosis factor a (TNFa) induced gap formation in human umbilical venous endothelial cells. CgA levels which reflect neuroendocrine differentiation of prostatic carcinoma may have a diagnostic, therapeutic and prognostic role in the management of prostate cancer patients.

Alternate Names

Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I

Gene Symbol

CHGA

UniProt

P10645

Additional Chromogranin A Products

Product Documents for Chromogranin A Antibody (SPM553)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Chromogranin A Antibody (SPM553)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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