Chromogranin A Antibody (SPM553)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32955
![Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody (SPM553) [NBP2-32955]](https://resources.bio-techne.com/images/products/Chromogranin-A-Antibody-SPM553-Immunocytochemistry-Immunofluorescence-NBP2-32955-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody (SPM553) [NBP2-32955]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM553
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human chromogranin A protein (Uniprot: P10645)
Localization
Finely granular cytoplasmic
Marker
Neuroendocrine Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34795)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Chromogranin A Antibody (SPM553)
Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody (SPM553) [NBP2-32955]
Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody (SPM553) [NBP2-32955] - SHSY5Y cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Chromogranin A conjugated to Alexa Fluor 488 [NBP2-34795AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the Alexa Fluor 488 version of this antibody.![Immunohistochemistry-Paraffin: Chromogranin A Antibody (SPM553) [NBP2-32955]](https://resources.bio-techne.com/images/products/Chromogranin-A-Antibody-SPM553-Immunohistochemistry-Paraffin-NBP2-32955-img0001.jpg "Immunohistochemistry-Paraffin: Chromogranin A Antibody (SPM553) [NBP2-32955]")
Immunohistochemistry-Paraffin: Chromogranin A Antibody (SPM553) [NBP2-32955]
Immunohistochemistry-Paraffin: Chromogranin A Antibody (SPM553) [NBP2-32955] - Formalin-fixed, paraffin-embedded human pancreas stained with chromograninn A Monoclonal Antibody (SPM553)Applications for Chromogranin A Antibody (SPM553)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Chromogranin A
Alternate Names
Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I
Gene Symbol
CHGA
UniProt
Additional Chromogranin A Products
Product Documents for Chromogranin A Antibody (SPM553)
Product Specific Notices for Chromogranin A Antibody (SPM553)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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