Chromogranin A Antibody (SPM553) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34795
![Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody (SPM553) - Azide and BSA Free [NBP2-34795]](https://resources.bio-techne.com/images/products/Chromogranin-A-Antibody-SPM553-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34795-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody (SPM553) - Azide and BSA Free [NBP2-34795]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM553
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human chromogranin A protein (Uniprot: P10645)
Localization
Finely granular cytoplasmic
Marker
Neuroendocrine Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32955).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Chromogranin A Antibody (SPM553) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody (SPM553) - Azide and BSA Free [NBP2-34795]
Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody (SPM553) - Azide and BSA Free [NBP2-34795] - SHSY5Y cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Chromogranin A conjugated to Alexa Fluor 488 [NBP2-34795AF488] at 10ug/ml for 1 hour at ro![Immunohistochemistry-Paraffin: Chromogranin A Antibody (SPM553) - Azide and BSA Free [NBP2-34795]](https://resources.bio-techne.com/images/products/Chromogranin-A-Antibody-SPM553-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34795-img0002.jpg "Immunohistochemistry-Paraffin: Chromogranin A Antibody (SPM553) - Azide and BSA Free [NBP2-34795]")
Immunohistochemistry-Paraffin: Chromogranin A Antibody (SPM553) - Azide and BSA Free [NBP2-34795]
Immunohistochemistry-Paraffin: Chromogranin A Antibody (SPM553) - Azide and BSA Free [NBP2-34795] - Formalin-fixed, paraffin-embedded human pancreas stained with chromogranin A Monoclonal Antibody (SPM553)Applications for Chromogranin A Antibody (SPM553) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Chromogranin A
Alternate Names
Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I
Gene Symbol
CHGA
Additional Chromogranin A Products
Product Documents for Chromogranin A Antibody (SPM553) - Azide and BSA Free
Product Specific Notices for Chromogranin A Antibody (SPM553) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...