Endoglin/CD105 Antibody (MJ7/18)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-77666

Novus Biologicals, part of Bio-Techne
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NB100-77666
NB100-77666SS
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat

Applications

Validated:

Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # MJ7/18

Concentration

1.0 mg/ml

View all Endoglin/CD105 Antibodies »

Product Specifications

Immunogen

Inflamed mouse skin

Epitope

Endothelial cell selective TGB1 binding molecule-endoglin

Marker

Neo-endothelial Cells Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for Endoglin/CD105 Antibody (MJ7/18)

Western Blot: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666]

Western Blot: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666]

Western Blot: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Total protein from mouse placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocking in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD105 (NB100-77666) in 1% milk/TBST and detected with an anti-rat HRP conjugated secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666]

Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666]

Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Using the DyLight 550 direct conjugate Staining of CD105 (endoglin) in 4T1 tumors after radioimmunotherapy shows CD105 expression (red), blood vessels (green), and nuclei (blue).
Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666]

Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666]

Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Analysis of a FFPE mouse ductus deference using a 1:50 dilution of CD105 antibody (clone MJ7/18) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Staining was observed in the pseudostratified columnar epithelial cells. Staining was performed by Histowiz.
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Applications for Endoglin/CD105 Antibody (MJ7/18)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume. It is recommended that the reagent be titrated for optimal performance for each application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Endoglin/CD105

CD105, commonly referred to as Endoglin, ENG, HHT1, and ORW1 is a membrane single-pass type I membrane protein limited to endothelial cells in all tissues except bone marrow. It is a major glycoprotein of vascular endothelium and plays a major role in the regulation of angiogenesis. CD105 acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. Mutations involving CD105 have been shown to be involved with hereditary hemorrhagic telangiectasia, as well interruptions of the TGF-beta/BMP pathways leading to some cancers. Immunohistochemistry has shown that CD105 has a very high expression in blood vessels of tumors, making it a potential target that can be used in tumor imaging (PMID: 12773481). Elevated serum levels of CD105 in pregnant women have been determined a possible risk factor for preeclampsia (PMID: 24273904).

Alternate Names

CD105, ENG

Gene Symbol

ENG

Additional Endoglin/CD105 Products

Product Documents for Endoglin/CD105 Antibody (MJ7/18)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Endoglin/CD105 Antibody (MJ7/18)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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