Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80718
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # MJ7/18
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Inflamed mouse skin
Epitope
Endothelial cell selective TGB1 binding molecule-endoglin
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Scientific Data Images for Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free
Western Blot: Endoglin/CD105 Antibody (MJ7/18)Azide and BSA Free [NBP2-80718]
Western Blot: Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718] - Total protein from mouse placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocking in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD105 (NB100-77666) in 1% milk/TBST and detected with an anti-rat HRP conjugated secondary antibody using chemiluminescence. Image from the standard format of this antibody.Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718]
Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718] - Using the DyLight 550 direct conjugate Staining of CD105 (endoglin) in 4T1 tumors after radioimmunotherapy shows CD105 expression (red), blood vessels (green), and nuclei (blue).Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718]
Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718] - Analysis of a FFPE mouse ductus deference using a 1:50 dilution of CD105 antibody (clone MJ7/18) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)Applications for Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume. It is recommended that the reagent be titrated for optimal performance for each application.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
Tris-Glycine
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Endoglin/CD105
Alternate Names
CD105, ENG
Gene Symbol
ENG
Additional Endoglin/CD105 Products
Product Documents for Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free
Product Specific Notices for Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...