Endoglin/CD105 Antibody (SN6)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65601

Clone SN6 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB100-65601
NB100-65601-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # SN6

Concentration

1 mg/ml

View all Endoglin/CD105 Antibodies »

Product Specifications

Immunogen

Partially purified cell membrane antigens from fresh leukemia cells

Reactivity Notes

Predicted cross-reactivities: Cynomolgus monkey, Equine, Primate, Rhesus Monkey

Specificity

Recognizes the human CD105 cell surface antigen, a glycoprotein homodimer of 95kD subunits. CD105 is also known as endoglin, and is expressed by endothelial cells, activated monocytes and some leukemia cells.

Marker

Neo-endothelial Cells Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Endoglin/CD105 Antibody (SN6)

Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]

Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]

Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - A surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (SN6) NB100-65601PE and a matched isotype control. Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]

Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]

Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 488
Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]

Flow Cytometry: Endoglin/CD105 Antibody (SN6) - BSA Free [NB100-65601]

Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 647
View 4 more images

Applications for Endoglin/CD105 Antibody (SN6)

Application
Recommended Usage

Flow Cytometry

1:50

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Endoglin/CD105

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

Alternate Names

CD105, ENG

Gene Symbol

ENG

UniProt

P17813

Additional Endoglin/CD105 Products

Product Documents for Endoglin/CD105 Antibody (SN6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Endoglin/CD105 Antibody (SN6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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