His Tag Antibody (RM146)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61482

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-61482
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Key Product Details

Species Reactivity

Epitope Tag

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM146

Concentration

1 mg/ml

View all His Tag Antibodies »

Product Specifications

Immunogen

This His Tag Antibody (RM146) was developed against a mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

Specificity

This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for His Tag Antibody (RM146)

Western Blot: His Tag Antibody (RM146) [NBP2-61482]

Western Blot: His Tag Antibody (RM146) [NBP2-61482]

Western Blot: His Tag Antibody (RM146) [NBP2-61482] - Western blot of 293T cells (1) untransfected or (2) transfected with His-Tag fusion protein X and (3) E. coli lysate without His-Tag Protein Y (4) E. coli lysate with His-Tag Protein Y, using NBP2-61482.
Immunohistochemistry: His Tag Antibody (RM146) [NBP2-61482]

Immunohistochemistry: His Tag Antibody (RM146) [NBP2-61482]

Immunohistochemistry: His Tag Antibody (RM146) [NBP2-61482] - Immunohistochemistry staining of naive HepG2 cells (Negative control) using anti-His-Tag antibody, NBP2-61482.
Immunohistochemistry-Paraffin: His Tag Antibody (RM146) [NBP2-61482]

Immunohistochemistry-Paraffin: His Tag Antibody (RM146) [NBP2-61482]

Immunohistochemistry-Paraffin: His Tag Antibody (RM146) [NBP2-61482] - Immunohistochemistry staining of 293T cells expressing His-Tag nuclear protein X, using NBP2-61482.
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Applications for His Tag Antibody (RM146)

Application
Recommended Usage

Flow Cytometry

0.5 ug/mL - 2 ug/mL

Immunocytochemistry/ Immunofluorescence

0.5 ug/mL - 2 ug/mL

Immunohistochemistry

0.1 ug/mL - 1 ug/mL

Immunoprecipitation

0.5 ug/ml - 2 ug/ml

Simple Western

reported from a verified customer review.

Western Blot

0.1 ug/ml - 1 ug/ml
Application Notes
His Tag Antibody (RM146) validated for Simple Western from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP2-61482 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: His Tag

A his-tag (also known as histidine tag or polyhistidine tag) is a common epitope tag that typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a targeted recombinant protein to facilitate its purification and detection (1). The most common his-tag is the hexahistidine (His6/6-His) tag which has a theoretical molecular weight of 0.8kda (1). The histidine residues readily interact with transition metal ions such as Co2+, Ni2+, Cu2+, and Zn2+, making immobilized metal-affinity chromatography (IMAC) the preferred technique for his-tag purification (1, 2). Metal ions are immobilized and bound to by His-tags in the IMAC column via the histidine imidazole ring. The tagged protein can be eluted off the column by washing with buffers containing a low concentration of imidazole (1, 2). Due to its relatively small size, low immunogenicity, versatility under denaturing conditions, and minimal interference with the structure and function of proteins, the his-tag is one of the most widely used tags for protein purification (1-3).

References

1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0

2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6

3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V., ... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598

Long Name

Histidine Tag

Alternate Names

polyHistidine

Additional His Tag Products

Product Documents for His Tag Antibody (RM146)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for His Tag Antibody (RM146)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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