His Tag Antibody (RM146) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26047
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Epitope Tag
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM146
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This His Tag Antibody (RM146) was developed against a mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
Specificity
This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for His Tag Antibody (RM146) - Azide and BSA Free
Western Blot: His Tag Antibody (RM146) - Azide and BSA Free [NBP3-26047] -
Western Blot: His Tag Antibody (RM146) - Azide and BSA Free [NBP3-26047] - Western blot of 293T cells (1) untransfected or (2) transfected with His Tag fusion protein X and (3) E. coli lysate without His Tag Protein Y (4) E. coli lysate with His Tag Protein Y, using RM146 at 0.2 ug/mL.
Immunohistochemistry-Paraffin: His Tag Antibody (RM146) - Azide and BSA Free [NBP3-26047] -
Immunohistochemistry-Paraffin: His Tag Antibody (RM146) - Azide and BSA Free [NBP3-26047] - Immunohistochemistry staining of 293T cells expressing His-Tag nuclear protein X, using Clone RM146.
Immunohistochemistry-Paraffin: His Tag Antibody (RM146) - Azide and BSA Free [NBP3-26047] -
Immunohistochemistry-Paraffin: His Tag Antibody (RM146) - Azide and BSA Free [NBP3-26047] - Immunohistochemistry staining of naive HepG2 cells (Negative control) using Clone RM146.Applications for His Tag Antibody (RM146) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 ug/mL - 2 ug/mL
Immunocytochemistry/ Immunofluorescence
0.5 ug/mL - 2 ug/mL
Immunohistochemistry
0.1 ug/mL - 1 ug/mL
Immunoprecipitation
0.5 ug/ml - 2 ug/ml
Simple Western
reported from a verified customer review.
Western Blot
0.1 ug/ml - 1 ug/ml
Application Notes
His Tag Antibody (RM146) validated for Simple Western from a verified customer review.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: His Tag
References
1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0
2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6
3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V., ... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598
Long Name
Histidine Tag
Alternate Names
polyHistidine
Additional His Tag Products
Product Documents for His Tag Antibody (RM146) - Azide and BSA Free
Product Specific Notices for His Tag Antibody (RM146) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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