His Tag Antibody (RM146) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61482B
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Epitope Tag
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Biotin
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM146
Concentration
1 mg/ml
Product Specifications
Immunogen
This His Tag Antibody (RM146) was developed against a mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
Specificity
This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for His Tag Antibody (RM146) [Biotin]
Western Blot: Rabbit anti-All Species His Tag Secondary Antibody (RM146) [Biotin] [NBP2-61482B] - Western blot of 293T cells transfected (+) or untransfected (-) with a DNA construct encoding His-Tag Histone H3 (G34W) protein, using Biotin Anti-HisTag Rabbit Monoclonal Antibody Clone RM146 at 0.2 ug/mL, followed by a HRP conjugated Streptavidin.
Immunohistochemistry: Rabbit anti-All Species His Tag Secondary Antibody (RM146) [Biotin] [NBP2-61482B] -
Immunohistochemistry: Rabbit anti-All Species His Tag Secondary Antibody (RM146) [Biotin] [NBP2-61482B] - Immunohistochemistry staining of 293T cells expressing His-Tag nuclear protein X, using unconjugated form of this antibody (NBP2-61482)
Immunohistochemistry: Rabbit anti-All Species His Tag Secondary Antibody (RM146) [Biotin] [NBP2-61482B] -
Immunohistochemistry: Rabbit anti-All Species His Tag Secondary Antibody (RM146) [Biotin] [NBP2-61482B] - Immunohistochemistry staining of naive HepG2 cells (Negative control) using unconjugated form of this antibody (NBP2-61482)Applications for His Tag Antibody (RM146) [Biotin]
Application
Recommended Usage
ELISA
0.01 ug/ml - 0.5 ug/ml
Flow Cytometry
0.5 ug/mL - 2 ug/mL
Immunocytochemistry/ Immunofluorescence
0.5 - 2 ug/ml
Immunohistochemistry
0.1 ug/mL - 1 ug/mL
Western Blot
0.1 ug/ml - 0.5 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 50% Glycerol, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: His Tag
References
1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0
2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6
3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V., ... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598
Long Name
Histidine Tag
Alternate Names
polyHistidine
Additional His Tag Products
Product Documents for His Tag Antibody (RM146) [Biotin]
Product Specific Notices for His Tag Antibody (RM146) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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