His Tag Horseradish Peroxidase-conjugated Antibody Best Seller
R&D Systems, part of Bio-Techne | Catalog # MAB050H
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Multi-Species
Cited:
Human, Mouse, Bacteria, Bacteria - Escherichia coli, Bacteria - Vibrio parahaemolyticus, E. coli, Parasite - Fasciola hepatica (liver fluke), Yeast
Applications
Validated:
Simple Western, Western Blot
Cited:
Binding Assay, Bioassay, ELISA Detection, ELISA Development, ELISA Development (Detection), Flow Cytometry, Functional Assay, Western Blot
Label
HRP
Antibody Source
Monoclonal Mouse IgG1 Clone # AD1.1.10
Product Specifications
Immunogen
His-tagged peptide
Specificity
Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags at the amino- or carboxyl‑terminus.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for His Tag Horseradish Peroxidase-conjugated Antibody
Detection of His Tag by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line either non-transfected or transfected with His-tagged human CIQ4 and CHO Chinese hamster ovary cell line transfected with His-tagged BAI-1. PVDF membrane was probed with a 1:4000 dilution of Mouse Anti-His Tag HRP-conjugated Monoclonal Antibody (Catalog # MAB050H). Specific bands were detected for His Tag at approximately 32 and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of His Tag by Simple WesternTM.
Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with His-tagged human CIQ4 and human MYCOC and CHO Chinese hamster ovary cell line transfected with His-tagged BAI-1, loaded at 0.2 mg/mL. Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated) using a 1:1000 dilution of Mouse Anti-His Tag HRP-conjugated Monoclonal Antibody (Catalog # MAB050H) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for His Tag Horseradish Peroxidase-conjugated Antibody
Application
Recommended Usage
Simple Western
1:1000 dilution
Sample: HEK293 human embryonic kidney cell line and CHO Chinese hamster ovary cell line transfected with His-tagged proteins
Sample: HEK293 human embryonic kidney cell line and CHO Chinese hamster ovary cell line transfected with His-tagged proteins
Western Blot
1:4000 dilution
Sample: HEK293 human embryonic kidney cell line and CHO Chinese hamster ovary cell line transfected with His-tagged proteins
Sample: HEK293 human embryonic kidney cell line and CHO Chinese hamster ovary cell line transfected with His-tagged proteins
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 6 reviews rated 5 using MAB050H in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied as a 0.2 μm filtered solution in Sodium Phosphate and Proclin 300. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack (-SP) is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Do not freeze.
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: His Tag
Long Name
Histidine Tag
Alternate Names
polyHistidine
Additional His Tag Products
Product Documents for His Tag Horseradish Peroxidase-conjugated Antibody
Product Specific Notices for His Tag Horseradish Peroxidase-conjugated Antibody
For research use only
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