NS0 mouse myeloma cell line transfected with His-tagged protein was stained with Mouse Anti-His Tag PerCP-conjugated Monoclonal Antibody (Catalog # IC050C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for His Tag PerCP-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: NS0 mouse myeloma cell line transfected His-tagged protein fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: His Tag

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Long Name

Histidine Tag

Alternate Names

polyHistidine

Additional His Tag Products

Product Documents for His Tag PerCP-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for His Tag PerCP-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

His Tag PerCP-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC050C

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all His Tag Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for His Tag PerCP-conjugated Antibody

Detection of His Tag in NS0 Mouse Cell Line Transfected with His-tagged Protein by Flow Cytometry.