HEK293 human embryonic kidney cell line transfected with human 5-HT4 and eGFP was stained with (A) Mouse Anti-Human 5-HT4 Monoclonal Antibody (Catalog # MAB6545) or (B) Mouse IgG2B isotype control antibody (IC0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.

5-HT4 in Human Brain.

5-HT4 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human 5-HT4 Monoclonal Antibody (Catalog # MAB6545) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human 5-HT4 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human 5-HT4 and eGFP

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 5-HT4

5-HT4 (5-hydroxytryptamine receptor 4), also known as HTR4 or 5HT4R, is an approximately 45 kDa multipass serotonin receptor that is expressed in the brain, gastrointestinal tract, bladder, vascular endothelial cells, atrium, kidney, and adrenal gland. It is involved in the regulation of learning, memory, anxiety, smooth muscle contraction, and adrenal corticosteroid secretion. Alternate splicing of human 5-HT4 generates multiple isoforms with either a 14 aa insertion in the second extracellular loop or a deletion or various substitutions in the C-terminal cytoplasmic region. Different isoforms show tissue specific expression patterns or increased activity in the absence of ligand. Human 5-HT4 shares 94% and 88% aa sequence identity with mouse and rat 5-HT4, respectively.

Long Name

5-Hydroxytryptamine Receptor 4

Alternate Names

5HT4, HTR4

Entrez Gene IDs

3360 (Human)

Gene Symbol

HTR4

Additional 5-HT4 Products

Product Documents for Human 5-HT4 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human 5-HT4 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human 5-HT4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6545

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all 5-HT4 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human 5-HT4 Antibody

Detection 5-HT4 in HEK293 Human Cell Line Transfected with Human 5-HT4 and eGFP by Flow Cytometry