Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 5D3

View all ABCG2 Antibodies »

Product Specifications

Immunogen

3T3 cells transduced with human ABCG2

Specificity

Detects human ABCG2 in flow cytometry and immunocytochemistry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human ABCG2 Alexa Fluor® 488-conjugated Antibody

Detection of ABCG2 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry.

Detection of ABCG2 in MCF-7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ABCG2 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB995G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human ABCG2 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: MCF‑7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ABCG2

Hematopoietic stem cells are known to express a membrane transporter molecule, known as P-glycoprotein (Pgp), that is encoded by the Multidrug Resistance Gene 1 (MDR1) (1, 2). Expression of Pgp appears to confer a proliferative advantage to stem cells through its anti-apoptotic effects (3, 4). An additional transporter molecule known as ABCG2 (ATP-Binding Cassette Gene 2) or Bcrp1 (Breast Cancer Resistance Protein 1), first identified in a breast cancer cell line (5), is expressed on stem cells (6). ABCG2 belongs to a family of molecules that span the cell membrane six times and can exist as either homo or hetero dimers linked by a short intracellular flexible linker region that plays an important role in the efflux of a wide range of substrates (7, 8). Although these transporter molecules have initially been thought to play a role in drug resistance, they have been found to have utility in better characterizing primitive stem cells. For example, the “side-population” of hematopoietic stem cells, characterized by their inability to retain high levels of the intracellular staining dyes Hoechst 33342 and Rhodamine 123, has been found to express high levels of ABCG2. Of interest is the observation that ABCG2 function has been linked to the efflux of the Hoechst dye (6). Furthermore, there is now evidence that this monoclonal can be used as a cell surface marker to identify hematopoietic stem cells within the bone marrow fraction of lineage negative cells (6). The expression of ABCG2 appears greatest on CD34- cells and is downregulated with the acquisition of CD34 on the cell surface (6).

References

  1. Chaudhary, P.M. and I.B. Roninson (1991) Cell 66:85.
  2. Sorrentino, B.P. et al. (1995) Blood 86:491.
  3. Pallis, M. and N. Russell (2000) Blood 95:2897.
  4. Johnstone, R.W. et al. (1999) Blood 93:1075.
  5. Doyle, L.A. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15665.
  6. Zhou, S. et al. (2001) Nat. Medicine 7:1028.
  7. Hrycyna, C.A. et al. (1998) Biochem. 37:13660.
  8. Bunting, K.D. (2002) Stem Cells 20:11.

Long Name

ATP-binding Cassette Transporter G2

Alternate Names

ABCP, BCRP, CD338, MXR

Entrez Gene IDs

9429 (Human)

Gene Symbol

ABCG2

Additional ABCG2 Products

Product Documents

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Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ABCG2 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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