ATP-Binding Cassette Transporter G2 (ABCG2) was detected in immersion fixed JAR human choriocarcinoma cell line using Mouse Anti-Human ABCG2 Monoclonal Antibody (Catalog # MAB995) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; NL009) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

ABCG2 in Human ABCG2 transfected CHO Chinese Hamster Cell Line.

ABCG2 was detected in immersion fixed Human ABCG2 transfected CHO Chinese hamster ovary cell line using Mouse Anti-Human ABCG2 Monoclonal Antibody (Catalog # MAB995) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

ABCG2 in A549 Human Cell Line.

ABCG2 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human ABCG2 Monoclonal Antibody (Catalog # MAB995) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Applications for Human ABCG2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human ABCG2 transfected CHO-S cells; RPMI 8226 human multiple myeloma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed JAR human choriocarcinoma cell line and Human ABCG2 transfected CHO Chinese hamster ovary cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ABCG2

Hematopoietic stem cells are known to express a membrane transporter molecule, known as P-glycoprotein (Pgp), that is encoded by the multidrug resistance gene 1 (MDR1) (1, 2). Expression of Pgp appears to confer a proliferative advantage to stem cells through its anti-apoptotic effects (3, 4). An additional transporter molecule known as ABCG2 (ATP-binding cassette gene 2) or Bcrp1 (Breast cancer resistance protein 1), first identified in a breast cancer cell line (5), is expressed on stem cells (6). ABCG2 belongs to a family of molecules that span the cell membrane six times and can exist as either homo or hetero dimers linked by a short intracellular flexible linker region that plays an important role in the efflux of a wide range of substrates (7, 8). Although these transporter molecules have initially been thought to play a role in drug resistance, they have been found to have utility in better characterizing primitive stem cells. For example, the “side-population” of hematopoietic stem cells, characterized by their inability to retain high levels of the intracellular staining dyes Hoechst 33342 and Rhodamine 123, has been found to express high levels of ABCG2. Of interest is the observation that ABCG2 function has been linked to the efflux of the Hoechst dye (6). Furthermore, there is now evidence that this monoclonal can be used as a cell surface marker to identify hematopoietic stem cells within the bone marrow fraction of lineage negative cells (6). The expression of ABCG2 appears greatest on CD34^- cells and is downregulated with the acquisition of CD34 on the cell surface (6).

References

Chaudhary, P.M. and I.B. Roninson (1991) Cell 66:85.
Sorrentino, B.P. et al. (1995) Blood 86:491.
Pallis, M. and N. Russell (2000) Blood 95:2897.
Johnstone, R.W. et al. (1999) Blood 93:1075.
Doyle, L.A. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15665.
Zhou, S. et al. (2001) Nat. Medicine 7:1028.
Hrycyna, C.A. et al. (1998) Biochem. 37:13660.
Bunting, K.D. (2002) Stem Cells 20:11.

Long Name

ATP-binding Cassette Transporter G2

Alternate Names

ABCP, BCRP, CD338, MXR

Entrez Gene IDs

9429 (Human)

Gene Symbol

ABCG2

Additional ABCG2 Products

Product Documents for Human ABCG2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ABCG2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human ABCG2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB995

Key Product Details

Species Reactivity

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Applications

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Label

Antibody Source

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Product Specifications

Immunogen

Specificity

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Host

Isotype

Scientific Data Images for Human ABCG2 Antibody

ABCG2 in JAR Human Cell Line.