Human Activin C Antibody

Name: Human Activin C Antibody
Brand: R&D Systems
SKU: MAB11501

R&D Systems, part of Bio-Techne | Catalog # MAB11501

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 1077920

View all Activin C Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Activin C
Gly237-Ser352
Accession # P55103

Specificity

Detects human Activin C in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human Activin C Antibody

Detection of Activin C in Human Liver.

Activin C was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Activin C Monoclonal Antibody (Catalog # MAB11501) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of liver hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Activin C in HepG2 cells (Positive) and THP-1 cells (Negative).

Activin C was detected in fixed HepG2 human hepatocellular carcinoma cell line (Positive) and absent in THP-1 human acute monocytic leukemia cell line (Negative) using Mouse Anti-Human Activin C Monoclonal Antibody (Catalog # MAB11501) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Activin C Antibody

Application

Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: fixed HepG2 human hepatocellular carcinoma cell line (Positive) and absent in THP‑1 human acute monocytic leukemia cell line (Negative)

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin C

Activins belong to the TGF-beta superfamily. They are homo- or heterodimers of Activin beta subunits. At least four mammalian beta subunits, A, B, C, and E, have been described. The beta_c subunit homodimerizes to form Activin CC. A naturally occuring Activin AC heterodimer has also been reported (1).

References

Mellor, S. et al. (2003) Endocrinology 144:4410.

Alternate Names

INHBC

Entrez Gene IDs

3626 (Human); 16325 (Mouse)

Gene Symbol