Human ADAMTS13 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4245
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS13
Gln34-Trp688
Accession # Q76LX8
Gln34-Trp688
Accession # Q76LX8
Specificity
Detects human ADAMTS13 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) ADAMTS14 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human ADAMTS13 Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with anti-Human ADAMTS13 monoclonal antibody (Catalog # MAB42451).This product is intended for assay development on various assay platforms requiring antibody pairs.
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAMTS13 Full Length (Catalog # 6156-AD), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAMTS13 Full Length (Catalog # 6156-AD), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human ADAMTS13 (Catalog # 4245-AD)
Sample: Recombinant Human ADAMTS13 (Catalog # 4245-AD)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAMTS13
ADAMTS13 (A disintegrin and metalloprotease with TSP motifs 13) is a 140-230 kDa, secreted glycoprotein member of the ADAMTS family of proteases. ADAMTS13 is produced by the liver and circulates in plasma where it cleaves and reduces the activity of von Willebrand factor. ProADAMTS13 is 1398 amino acids (aa) in length. It contains a 45 aa prodomain (aa 30-74), one peptidase domain (aa 80-286) and one disintegrin (aa 287-383) domain, multiple TSP regions and two CUB domains. The prodomain does not affect activity, while the TSP and CUB domains appear to be necessary for activity. One truncated form exists that shows truncation after Gln448. Over aa 34-688, human ADAMST13 shares 76% aa identity with mouse ADAMST13.
Long Name
A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 13
Alternate Names
TTP, vWF-CP
Gene Symbol
ADAMTS13
UniProt
Additional ADAMTS13 Products
Product Documents for Human ADAMTS13 Antibody
Product Specific Notices for Human ADAMTS13 Antibody
For research use only
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