Human Apolipoprotein B/ApoB Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB41241
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 989529
Product Summary for Human Apolipoprotein B/ApoB Antibody
Immunogen
E. coli-derived recombinant human Apolipoprotein B/ApoB
Met1206-Asp1413
Accession # NP_000375
Met1206-Asp1413
Accession # NP_000375
Specificity
Detects human Apolipoprotein B/ApoB in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Apolipoprotein B/ApoB Antibody
Apolipoprotein B/ApoB in Human HepG2 and MCF-7 Cell Lines.
Apolipoprotein B/ApoB was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive control, left panel) and MCF-7 human breast cancer cell line (negative control, right panel) using Mouse Anti-Human Apolipoprotein B/ApoB Monoclonal Antibody (Catalog # MAB41241) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Apolipoprotein B/ApoB in Human Liver.
Apolipoprotein B/ApoB was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Apolipoprotein B/ApoB Monoclonal Antibody (Catalog # MAB41241) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Apolipoprotein B/ApoB Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line and MCF‑7 human breast cancer cell line
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line and MCF‑7 human breast cancer cell line
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Apolipoprotein B/ApoB
Human ApoB (Apolipoprotein B-100) is a 550 kDa, secreted, palmitoylated glycoprotein that is part of LDL and VLDL particles. It is made by liver and is 4536 amino acids (aa) in length. It binds LDL to the ApoB/E receptor. Multiple forms of ApoB-100 exist; ApoB-25, 26, 29, 37, 39, 48, and 74. The numbers reflect each molecule’s predicted MW as a percentage of ApoB-100 (512 kDa). Amino acids 1206-1414 of the precursor were used for immunization. Based on likelihood of secretion, this antibody may recognize B26 (1297 aa), B37 (1728 aa), B39 (1799 aa), and B48 (2152 aa). Over the immunization polypeptide range, human ApoB-100 is 80% and 82% aa identical to mouse and dog ApoB-100, respectively.
Alternate Names
APOB, ApoB-100, ApoB-48, FLDB, LDLCQ4
Gene Symbol
APOB
UniProt
Additional Apolipoprotein B/ApoB Products
Product Documents for Human Apolipoprotein B/ApoB Antibody
Product Specific Notices for Human Apolipoprotein B/ApoB Antibody
For research use only
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