Human B7-H3 Antibody

Name: Human B7-H3 Antibody
Brand: R&D Systems
SKU: MAB11611

R&D Systems, part of Bio-Techne | Catalog # MAB11611

Submit a review

Product Datasheet / COA / SDS

Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line, NS0-derived human B7-H3 Leu29-Pro245 Accession # Q5ZPR3
Applications:	Immunohistochemistry, Multiplex Immunofluorescence, Simple Western, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2A Clone # 1090036
Specificity:	Detects recombinant protein specific for human B7-H3 in Direct ELISA.

View Full Technical Details for Human B7-H3 Antibody

Technical Data
Citations / Reviews

Applications for Human B7-H3 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	U2OS human osteosarcoma cell line
Simple Western	20 µg/mL	LNCaP human prostate cancer cell line
Immunohistochemistry	3-25 µg/mL	Immersion fixed paraffin-embedded sections of human lung cancer
Multiplex Immunofluorescence	20 µg/mL	Immersion fixed paraffin-embedded sections of human Lung Cancer

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human B7-H3 Antibody

	Multiplex Immunofluorescence Detection of B7-H3 in Human Lung Cancer via Multiplex Immunofluorescence staining on COMET™ B7-H3 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Mouse Anti-Human B7-H3 Monoclonal Antibody (Catalog # MAB11611) at 20ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
	Western Blot Detection of Human B7‑H3 by Western Blot. Western Blot shows lysates of U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/ml of Mouse Anti-Human B7‑H3 Monoclonal Antibody (Catalog # MAB11611) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for B7‑H3 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
	Immunohistochemistry Detection of B7‑H3 in Human Lung Cancer. B7‑H3 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Mouse Anti-Human B7‑H3 Monoclonal Antibody (Catalog # MAB11611) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
	Simple Western Detection of Human B7‑H3 by Simple Western^TM. Simple Western shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/ml. A specific band was detected for B7‑H3 at approximately 161 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human B7‑H3 Monoclonal Antibody (Catalog # MAB11611). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: B7-H3

Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for regulating immune responses (1‑3). Other family members include B7-1, B7-2, B7-H2, PD-L1 (B7-H1), and PD-L2. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share about 20‑40% amino acid (aa) sequence identity. The cloned human B7-H3 cDNA encodes a 316 aa type I membrane precursor protein with a putative 28 aa signal peptide, a 217 aa extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a 45 aa cytoplasmic domain. An isoform of human B7-H3 containing a four-Ig-like domain extracellular region has also been identified. Human B7-H3 is not expressed on resting B cells, T cells, monocytes or dendritic cells, but is induced on dendritic cells and monocytes by inflammatory cytokines. B7-H3 expression is also detected on various normal tissues and in some tumor cell lines. Human B7-H3 does not bind any known members of the CD28 family of immunoreceptors. However, B7-H3 has been shown to bind an unidentified counter-receptor on activated T cells to costimulate the proliferation of CD4+ or CD8+ T cells. B7-H3 has also been found to enhance the induction of primary cytotoxic T lymphocytes and stimulate IFN-gamma production (1‑3).

Item	Value
References	Chapoval, A.I. et al. (2001) Nat. Immunol. 2:269. Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
Long Name	B7 Homolog 3
Entrez Gene IDs	80381 (Human); 102657 (Mouse); 315716 (Rat); 102131295 (Cynomolgus Monkey)
Alternate Names	B7-H3, B7-H3B7 homolog 3, B7H3, B7H34Ig-B7-H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule

Customer Reviews for Human B7-H3 Antibody

There are currently no reviews for this product. Be the first to review Human B7-H3 Antibody and earn rewards!

Have you used Human B7-H3 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review

Human B7-H3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11611

Key Product Details

Applications for Human B7-H3 Antibody

Scientific Data Examples for Human B7-H3 Antibody

Multiplex Immunofluorescence

Western Blot

Immunohistochemistry

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: B7-H3

Customer Reviews for Human B7-H3 Antibody

Product Documents for Human B7-H3 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

Stay Connected