HEK293 human embryonic kidney cell line transfected with either (A) human B7-H7/HHLA2 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human B7-H7/HHLA2 Monoclonal Antibody (Catalog # MAB80841) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

B7-H7/HHLA2 in Human PBMCs.

B7-H7/HHLA2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human B7-H7/HHLA2 Monoclonal Antibody (Catalog # MAB80841) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

TMIGD2/CD28H Binding to B7-H7/ HHLA2-transfected HEK293 Human Cell Line is Blocked by Human B7-H7/HHLA2 Antibody.

In a functional flow cytometry test, biotinylated recombinant human TMIGD2/ CD28H (10 ng/mL, Catalog # 8316-TR) binds to HEK293 human embryonic kidney cell line transfected with human B7-H7/ HHLA2 (black dotted line). Binding is completely blocked (orange histogram) by 2.5 µg/mL of Mouse Anti-Human B7-H7/ HHLA2 Monoclonal Antibody (Catalog # MAB80841). Mouse IgG1 Isotype Control (Catalog # MAB002) at 2.5 µg/mL was used as a control (blue line). Cells were stained with Streptavidin-APC (Catalog # F0050).

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Applications for Human B7-H7/HHLA2 Antibody

Application

Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 100-300 ng/mL of Mouse anti-Human B7-H7/HHLA2 Antibody (Catalog # MAB80841) will block 50% of the binding of 500 ng/mL of Recombinant Human B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7) to immobilized Recombinant Human TMIGD2/CD28H Fc Chimera (Catalog # 8316-TR) coated at 125 ng/mL
(100 µL/well). At 1.25 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK Human Cell Line Transfected with Human B7-H7/HHLA2 and eGFP

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-H7/HHLA2

B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins (1, 2). Mature human B7-H7 consists of a 322 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 49 aa cytoplasmic domain (3-5).
B7‑H7 is constitutively expressed on monocytes and is up-regulated by LPS and IFN-gamma stimulation. It is expressed on LPS/IFN-gamma treated B cells but not on resting B cells (5). B7-H7 binds to cell surface determinants on resting and mature T cells, B cells, and monocytes as well as on immature and mature dendritic cells (5). Soluble B7-H7 inhibits the proliferation of activated CD4⁺ and CD8⁺ T cells and their production of IFN-gamma, TNF-alpha, IL-5, IL-10, IL-13, IL-17A, and IL-22 (5).

References

Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
Mager, D.L. et al. (1999) Genomics 59:255.
Flajnik, M.M. et al. (2012) Immunogenetics 64:571.
Zhao, R. et al. (2013) Proc. Natl. Acad. Sci. USA 110:9879.

Long Name

B7 Homolog 7

Alternate Names

B7H7, HHLA2

Entrez Gene IDs

11148 (Human); 102137704 (Cynomolgus Monkey)

Gene Symbol

HHLA2

UniProt

Q9UM44

Additional B7-H7/HHLA2 Products

Product Documents for Human B7-H7/HHLA2 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human B7-H7/HHLA2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human B7-H7/HHLA2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB80841

Key Product Details

Species Reactivity

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Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Endotoxin Level

Scientific Data Images for Human B7-H7/HHLA2 Antibody

Detection of B7-H7/HHLA2 in HEK Human Cell Line Transfected with Human B7-H7/HHLA2 and eGFP by Flow Cytometry.