PC-3 human prostate cancer cell line was stained with Mouse Anti-Human BMPR-IB/ ALK-6 Monoclonal Antibody (Catalog # MAB5051, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

Detection of BMPR-IB/ALK-6 in Human iPS cells differentiated to Mesoderm by Flow Cytometry.

Human iPS cells differentiated to mesoderm (using Catalog # SC030B) were stained with Mouse Anti-Human BMPR-IB/ALK-6 Monoclonal Antibody (Catalog # MAB5051, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibosy (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

BMPR‑IB/ALK‑6 in PC‑3 Human Cell Line.

BMPR-IB/ALK-6 was detected in immersion fixed PC-3 human prostate cancer cell line using Mouse Anti-Human BMPR-IB/ALK-6 Monoclonal Antibody (Catalog # MAB5051) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human BMPR-IB/ALK-6 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: PC‑3 human prostate cancer cell line and human iPS cells differentiated to mesoderm (using Catalog # SC030B)

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed PC-3 human prostate cancer cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB5051 in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMPR-IB/ALK-6

Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor IB (BMPR-IB), also known as activin receptor-like kinase (ALK)-6, is one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-beta family cytokines. In contrast to the TGF-beta receptor system in which the type I receptor does not bind TGF-beta in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IB binds BMP-4 with high-affinity in solution and is a potent BMP-4 antagonist in vitro. BMPR-IB is expressed in various tissues during embryogenesis. In adult tissues, BMPR-IB is only found in the brain. The extracellular domain of BMPR-IB shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IB are highly conserved and share 98% amino acid sequence identity.

References

Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
Ebendal, T. et al. (1998) J. Neuroscience Research 51:139.

Long Name

Bone Morphogenetic Protein Receptor IB

Alternate Names

ALK-6, BMPR1B, BMPRIB, CDw293

Entrez Gene IDs

658 (Human); 12167 (Mouse)

Gene Symbol

BMPR1B

UniProt

O00238

Additional BMPR-IB/ALK-6 Products

Product Documents for Human BMPR-IB/ALK-6 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human BMPR-IB/ALK-6 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human BMPR-IB/ALK-6 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5051

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human BMPR-IB/ALK-6 Antibody

Detection of BMPR-IB/ALK-6 in PC-3 Human Cell Line by Flow Cytometry.