Human Cdk6 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11636
Key Product Details
| Species Reactivity: | Human |
| Immunogen: |
E. coli-derived recombinant human CDK-6 Met1-Ala326 Accession # Q00534 |
| Applications: | Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot |
| Label: | Unconjugated |
| Source: | Monoclonal Mouse IgG2B Clone # 1092920 |
| Specificity: |
Detects recombinant human CDK-6 protein in Direct ELISA. |
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Western BlotDetection of Human Cdk6 by Western Blot. Western Blot shows lysates of Jurkat human acute T cell leukemia cell line and SUP‑T1 human T cell lymphoblastic lymphoma cell line. PVDF membrane was probed with 0.5 µg/ml of Mouse Anti-Human Cdk6 Monoclonal Antibody (Catalog # MAB11636) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cdk6 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1. |
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ImmunohistochemistryDetection of Cdk6 in Human Lung Cancer. Cdk6 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Mouse Anti-Human Cdk6 Monoclonal Antibody (Catalog # MAB11636) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
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ImmunohistochemistryDetection of Cdk6 in Human Pancreatic Cancer. Cdk6 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer using Mouse Anti-Human Cdk6 Monoclonal Antibody (Catalog # MAB11636) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
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ImmunocytochemistryDetection of Cdk6 in HepG2 Human Cell Line. Cdk6 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Cdk6 Monoclonal Antibody (Catalog # MAB11636) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
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Simple WesternDetection of Human Cdk6 by Simple WesternTM. Simple Western shows lysates of Jurkat human acute T cell leukemia cell line and SUP‑T1 human T cell lymphoblastic lymphoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Cdk6 at approximately 41 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Cdk6 Monoclonal Antibody (Catalog # MAB11636). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
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