HEK293 human embryonic kidney cell line transfected with either (A) human C5aR or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human C5aR Monoclonal Antibody (Catalog # MAB36481) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Complement Component C5aR1 in U937 Human Cell Line.

Complement Component C5aR1 was detected in immersion fixed U937 human histiocytic lymphoma cell line (positive) and SH-SY5Y human neuroblastoma cell line (negative control) using Mouse Anti-Human Complement Component C5aR1 Monoclonal Antibody (Catalog # MAB36481) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Complement Component C5aR1 in Human Spleen.

Complement Component C5aR1 was detected in immersion fixed paraffin-embedded sections of human spleen tissue using Mouse Anti-Human Complement Component C5aR1 Monoclonal Antibody (Catalog # MAB36481) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Complement Component C5aR1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human C5aR and eGFP

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen tissue

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C5aR1

Complement Component C5a R1 (C5aR1), also known as C5a ligand and CD88, is a 7TM protein expressed on myeloid, endothelial, epithelial, and smooth muscle cells. C5aR binds the activated complement anaphylatoxin C5a. In established allergic environments, this triggers neutrophil and eosinophil chemotaxis and the release of proinflammatory mediators. In contrast, C5aR1/C5a interactions are protective during allergen sensitization. Human C5aR1 shares 66% amino acid sequence identity with mouse and rat C5aR.

Long Name

Complement Component C5a Receptor 1

Alternate Names

C5AR1, CD88, Complement Component C5a R1

Entrez Gene IDs

728 (Human); 12273 (Mouse)

Gene Symbol

C5AR1

UniProt

P21730

Additional Complement Component C5aR1 Products

Product Documents for Human Complement Component C5aR1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Complement Component C5aR1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Complement Component C5aR1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB36481

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all Complement Component C5aR1 Antibodies »

Product Summary for Human Complement Component C5aR1 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Complement Component C5aR1 Antibody

Detection of C5aR in HEK293 Human Cell Line Transfected with Human C5aR and eGFP by Flow Cytometry.