Human DISP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3549
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 768213
Product Specifications
Immunogen
E. coli-derived recombinant human DISP1
Glu212-Lys492 and Ser746-Thr984
Accession # Q96F81
Glu212-Lys492 and Ser746-Thr984
Accession # Q96F81
Specificity
Detects human DISP1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant
human (rh) DISP2 or rhSMAGP is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human DISP1 Antibody
DISP1 in BG01V Human Embryonic Stem Cells.
DISP1 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human DISP1 Monoclonal Antibody (Catalog # MAB3549) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to membrane structures and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human DISP1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells
Sample: Immersion fixed BG01V human embryonic stem cells
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DISP1
DISP1 is a multispan (12) transmembrane protein with cytoplasmic N- and C-terminal tails. It contains a stero/sensing domain (SSD) and has been shown to play a role in regulating the release of cholesterol-modified SHH. Within the regions used for immunization, human and mouse DISP1 share 93% amino acid sequence homology.
Long Name
Dispatched 1
Alternate Names
DISPA, Dispatched
Gene Symbol
DISP1
UniProt
Additional DISP1 Products
Product Documents for Human DISP1 Antibody
Product Specific Notices for Human DISP1 Antibody
For research use only
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