Human E6AP/UBE3A Antibody

Name: Human E6AP/UBE3A Antibody
Brand: R&D Systems
SKU: MAB11546

R&D Systems, part of Bio-Techne | Catalog # MAB11546

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Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody.

Key Product Details

Species Reactivity:	Human
Applications:	Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Label:	Unconjugated
Specificity:	Detects recombinant human His6-E6AP/UBE3A in Direct ELISA.

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Technical Data
Citations / Reviews

Applications for Human E6AP/UBE3A Antibody

	Recommended Concentration	Sample
Immunohistochemistry	3-25 µg/mL	Immersion fixed paraffin-embedded sections of human liver
Immunocytochemistry	3-25 µg/mL	fixed A172 human glioblastoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human E6AP/UBE3A Antibody

	Immunohistochemistry Detection of E6AP/UBE3A in Human Liver. E6AP/UBE3A was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human E6AP/UBE3A Monoclonal Antibody (Catalog # MAB11546) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Immunocytochemistry Detection of E6AP/UBE3A in A172 Human Cell Line. E6AP/UBE3A was detected in fixed A172 human glioblastoma cell line using Mouse Anti-Human E6AP/UBE3A Monoclonal Antibody (Catalog # MAB11546) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm and nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Formulation, Preparation and Storage

Reconstitution	Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Stability & Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: E6AP/UBE3A

Ubiquitin-protein ligase E3A, also known as E6AP, is an E3 ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several key substrates for UBE3A have been identified, including BMAL1, the PML tumor suppressor, PGR, and p53/TP53 suggesting a role for UBE3A in regulation of the circadian clock, tumor regulation, transcriptional coactivation of the progesterone receptors, and regulation of neoplastic progression of cells infected by high-risk human papilloma virus (1-4). Defects in activity are linked to Angelman syndrome, a neurodevelopmental disorder, as well as autism spectrum disorders implicating a role for UBE3A in regulation of neurobiological functions (3, 5, 6).

Item	Value
References	Dhananjayan, S.C. et. al. (2006) Mol. Endocrinol. 20:2343. Louria-Hayon, I. et. al. (2009) Cell Death Differ. 16:1156. Martinez-Noel, G. et. al. (2012) Mol. Cell. Biol. 32:3095. Gossan, N.C. et. al. (2014) Nucleic Acids Res. 42:5765. Sadikovic, B. et. al. (2014) Hum. Mutat. 35:1407. Khatri, N. and H-Y Man. (2019) Front. Mol. Neurosci. 12:109.
Long Name	Human Papillomavirus E6-associated Protein
Entrez Gene IDs	7337 (Human); 22215 (Mouse); 361585 (Rat)
Alternate Names	ANCR, ANCREPVE6AP, AS, CTCL tumor antigen se37-2, E6-AP, E6AP, E6AP ubiquitin-protein ligase, EC 6.3.2.-, EPVE6AP, FLJ26981, HPVE6A, Human papillomavirus E6-associated protein, Oncogenic protein-associated protein E6-AP, Renal carcinoma antigen NY-REN-54, UBE3A, human papilloma virus E6-associated protein, ubiquitin protein ligase E3A, ubiquitin-protein ligase E3A

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Human E6AP/UBE3A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11546

Key Product Details

Applications for Human E6AP/UBE3A Antibody

Scientific Data Examples for Human E6AP/UBE3A Antibody

Immunohistochemistry

Immunocytochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: E6AP/UBE3A

Customer Reviews for Human E6AP/UBE3A Antibody

Product Documents for Human E6AP/UBE3A Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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