Human FKBP12 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB37771
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1049713
Product Specifications
Immunogen
E. coli-derived human FKBP12F36V protein
Gly1-Glu108
Accession # P62942
Gly1-Glu108
Accession # P62942
Specificity
Detects both Human FKBP12 and FKBP12F36V mutant in direct Elisas
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human FKBP12 Antibody
FKBP12 in A431 human cell line.
FKBP12 was detected in immersion fixed A431 human epidermoid carcinoma cell line using Mouse Anti-Human FKBP12 Monoclonal Antibody (Catalog # MAB37771) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Adherent Cells.
Detection of Human, Mouse, and Rat FKBP12 by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human FKBP12 Monoclonal Antibody (Catalog # MAB37771) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for FKBP12 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Specificity of FKBP12 Shown by Western Blot.
Western blot shows recombinant human FKBP(F36V), FKBP12, and FKBP 12.6. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human FKBP12 Monoclonal Antibody (Catalog # MAB37771) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for FKBP(F36V) and FKBP12 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human FKBP12 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed A431 human epidermoid carcinoma cell line
Sample: Immersion fixed A431 human epidermoid carcinoma cell line
Western Blot
1 µg/mL
Sample: MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and rat brain tissue
Sample: MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and rat brain tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FKBP12
Long Name
12 kDa FK506 Binding Protein
Alternate Names
FKBP1a, PKC12, PPIASE
Gene Symbol
FKBP1A
UniProt
Additional FKBP12 Products
Product Documents for Human FKBP12 Antibody
Product Specific Notices for Human FKBP12 Antibody
For research use only
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