Human FOXL2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10396
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1019104
Product Specifications
Immunogen
Synthetic peptide containing human FOXL2
Accession # P58012
Accession # P58012
Specificity
Detects human FOXL2 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human FOXL2 Antibody
FOXL2 in PANC-1 Human Cell Line.
FOXL2 was detected in immersion fixed PANC-1 human pancreatic carcinoma cell line (positive) and MCF-7 human breast cancer cell line (negative) using Mouse Anti-Human FOXL2 Monoclonal Antibody (Catalog # MAB10396) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
FOXL2 in Ovarian Cancer Tissue.
FOXL2 was detected in immersion fixed paraffin-embedded sections of ovarian cancer tissue using Mouse Anti-Human FOXL2 Monoclonal Antibody (Catalog # MAB10396) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human FOXL2 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed PANC-1 human pancreatic carcinoma cell line (positive) and MCF‑7 human breast cancer cell line (negative)
Sample: Immersion fixed PANC-1 human pancreatic carcinoma cell line (positive) and MCF‑7 human breast cancer cell line (negative)
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of ovarian cancer tissue
Sample: Immersion fixed paraffin-embedded sections of ovarian cancer tissue
Reviewed Applications
Read 1 review rated 5 using MAB10396 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FOXL2
Long Name
Forkhead Box L2
Alternate Names
BPES, BPES1, PFRK, PINTO, POF3
Gene Symbol
FOXL2
UniProt
Additional FOXL2 Products
Product Documents for Human FOXL2 Antibody
Product Specific Notices for Human FOXL2 Antibody
For research use only
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