HEK293 human embryonic kidney cell line transfected with either (A) human GFRa-like (filled histogram) or (B) irrelevant transfectants (open histogram) was stained with Rabbit Anti-Human GFRa-like Monoclonal Antibody (Catalog # MAB9697) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Applications for Human GFR alpha-like Antibody

Application

Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA binding assay, 100-800 ng/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Human GDF-15 (Catalog # 9279-GD) to immobilized Recombinant Human GFR alpha-like Fc Chimera (Catalog # 9697-GR) coated at 0.1 μg/mL (100 μL/well). At 10 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human GFR alpha-like

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-like

GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins (1). Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain. Over the extracellular domain, human GFRAL shares 72% and 71% identity with mouse and rat GFRAL respectively. It is expressed on both fetal and adult hindbrain neurons of the CNS (3), and would appear to function as an anti-apoptotic molecule during neuronal stress. GFRAL is a functional receptor for GDF-15, facilitating weight-loss functions of the protein through c-Ret downstream signaling (2-4). GFRAL and GDF-15 signaling is implicated in diet-based obesity and insulin resistance (2-4).

References

Li, Z. et al. (2005) J. Neurochem. 95:361.
Mullican, S. et al. (2017) Nat. Med 23:1150.
Yang, L. et al. (2017) Nat. Med 23: 1158.
Emmerson, P. et al (2017) Nat. Med 23:1215.

Long Name

Glial Cell line-derived Neurotrophic Factor Receptor alpha-like

Alternate Names

GFR alpha like, GFRAL, GRAL

Entrez Gene IDs

389400 (Human); 404194 (Mouse); 501023 (Rat)

Gene Symbol

GFRAL

UniProt

Q6UXV0

Additional GFR alpha-like Products

Product Documents for Human GFR alpha-like Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human GFR alpha-like Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human GFR alpha-like Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9697

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all GFR alpha-like Antibodies »

Product Summary for Human GFR alpha-like Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human GFR alpha-like Antibody

Detection of GFR alpha-like in HEK293 Human Cell Line Transfected with Human GFR alpha-like by Flow Cytometry.