Human Glut1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11601
Key Product Details
| Species Reactivity: | Human |
| Immunogen: |
Synthetic Peptide Accession # P11166 |
| Applications: | Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot |
| Label: | Unconjugated |
| Source: | Monoclonal Mouse IgG2A Clone # 1084409 |
| Specificity: |
Detects a synthetic peptide corresponding to residues near amino acid 50 of human GLUT1 protein by Direct ELISA. |
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Western BlotDetection of Human Glut1 by Western Blot. Western Blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB11601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glut1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1. |
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ImmunocytochemistryDetection of Glut1 in HepG2 Human Cell Line. Glut1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line and absent in human PBMC cells using Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB11601) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cell surface of HepG2 cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
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ImmunohistochemistryDetection of Glut1 in Human Lung Cancer. Glut1 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB11601) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
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ImmunohistochemistryDetection of Glut1 in Human Pancreatic Cancer. Glut1 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer using Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB11601) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
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