Human GRK3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4785
Key Product Details
Species Reactivity
Human
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human GRK3
Pro469-Leu688
Accession # P35626
Pro469-Leu688
Accession # P35626
Specificity
Detects human GRK3 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human GRK3 Antibody
Detection of Human GRK3 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human GRK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4785) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GRK3 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human GRK3 by Simple WesternTM.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line, loaded at 0.2 mg/mL. A specific band was detected for GRK3 at approximately 80-81 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human GRK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4785) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human GRK3 Antibody
Application
Recommended Usage
Simple Western
20 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line
Sample: Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line
Sample: Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GRK3
Long Name
G protein-coupled Receptor Kinase 3
Alternate Names
ADRBK2, BARK2, Beta-ARK-2
Gene Symbol
GRK3
UniProt
Additional GRK3 Products
Product Documents for Human GRK3 Antibody
Product Specific Notices for Human GRK3 Antibody
For research use only
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