Human ICAM-1/CD54 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BBA9

R&D Systems, part of Bio-Techne
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BBA9
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Adhesion Blockade, Bioassay, Flow Cytometry, Functional Assay

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # BBIG-I1 (11C81)

View all ICAM-1/CD54 Antibodies »

Product Specifications

Immunogen

Activated human umbilical vein endothelial cells (HUVEC)

Specificity

Detects human ICAM-1 in Western blots. In this format, this antibody does not cross‑react with rhCEACAM-1, rhICAM-2, -3, -5, rmICAM-2, rrICAM-1, rmMAdCAM, or rhVCAM-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human ICAM-1/CD54 Biotinylated Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human ICAM‑1/CD54 Fc Chimera (Catalog # 720-IC)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using BBA9 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-1/CD54

ICAM-1 is a member of the immunoglobulin superfamily whose expression is upregulated on leukocytes, epithelial cells, and resting endothelial cells in response to inflammatory signals. ICAM-1 binds the leukocyte integrins LFA-1 and Mac-1.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Entrez Gene IDs

3383 (Human); 15894 (Mouse); 25464 (Rat)

Gene Symbol

ICAM1

Additional ICAM-1/CD54 Products

Product Documents for Human ICAM-1/CD54 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ICAM-1/CD54 Biotinylated Antibody

For research use only

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