Human ICAM-1/CD54 Antibody Best Seller
R&D Systems, part of Bio-Techne | Catalog # BBA3
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat
Applications
Validated:
Adhesion Blockade, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Cited:
Bead-based Bioassay, Bioassay, Cell-based ELISA, ELISA Capture, ELISA Development, ELISA Development (Capture), Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Inhibition, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # BBIG-I1 (11C81)
Product Specifications
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Specificity
This antibody was selected for its ability to stain human ICAM-1/CD54 transfected COS cells. It does not stain COS cells transfected with human E-Selectin, VCAM-1, or PECAM-1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human ICAM-1/CD54 Antibody
ICAM-1/CD54 in C32 Human Cell Line.
ICAM-1/CD54 was detected in live unfixed C32 human melanoma cell line using Mouse Anti-Human ICAM-1/CD54 Monoclonal Antibody (Catalog # BBA3) at 5 µg/mL for 1 hour at 2° - 8° C. Cells were then fixed with 4% formaldehyde followed by incubation with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm.
Detection of ICAM‑1/CD54 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human ICAM-1/CD54 Monoclonal Antibody (Catalog # BBA3) or (B) Mouse IgG1 Isotype Control (MAB002) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B) and Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (FAB3832A). Staining was performed using our Staining Membrane-associated Proteins protocol.
Detection of Human ICAM-1/CD54 by Western Blot.
Western blot shows lysates of C32. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ICAM-1/CD54 Monoclonal Antibody (Catalog # BBA3) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ICAM-1/CD54 at approximately 85 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.Applications for Human ICAM-1/CD54 Antibody
Application
Recommended Usage
Adhesion Blockade
The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Human ICAM-1/CD54 Fc Chimera (Catalog # 720-IC,12.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 10 µg/mL of the antibody.
Flow Cytometry
0.25 µg/106 cells
Sample: Human PBMC
Sample: Human PBMC
Immunocytochemistry
5-25 µg/mL
Sample: Live, unfixed C32 human melanoma cell line
Sample: Live, unfixed C32 human melanoma cell line
Immunoprecipitation
Davies, M.E., et al. (1991) Connect. Tissue Res. 26:207.
Western Blot
2 µg/mL
Sample: C32 human melanoma cell line
Sample: C32 human melanoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 6 reviews rated 4.8 using BBA3 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-1/CD54
Long Name
Intercellular Adhesion Molecule 1
Alternate Names
CD54, ICAM1
Gene Symbol
ICAM1
Additional ICAM-1/CD54 Products
Product Documents for Human ICAM-1/CD54 Antibody
Product Specific Notices for Human ICAM-1/CD54 Antibody
For research use only
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