Human ICAM-4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7179
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ICAM-4
Ala31-Ala240
Accession # Q14773
Ala31-Ala240
Accession # Q14773
Specificity
Detects human ICAM-4 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human ICAM-4 Antibody
Detection of Human ICAM‑4 by Western Blot.
Western blot shows lysates of human erythrocytes and human heart tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human ICAM-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7179) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ICAM-4 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human ICAM-4 by Simple WesternTM.
Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for ICAM-4 at approximately 48 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human ICAM-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7179) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of ICAM-4 in Human Red Blood Cells by Flow Cytometry.
Human red blood cells (erythrocytes) were stained with Sheep Anti-Human ICAM-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7179, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).Applications for Human ICAM-4 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human red blood cells (erythrocytes)
Sample: Human red blood cells (erythrocytes)
Simple Western
10 µg/mL
Sample: Human heart tissue
Sample: Human heart tissue
Western Blot
0.2 µg/mL
Sample: Human erythrocytes and human heart tissue
Sample: Human erythrocytes and human heart tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-4
ICAM-4 (intercellular adhesion molecule 4; also Landsteiner-Wiener glycoprotein and CD242) is a 42 kDa member of the ICAM family, Ig superfamily of proteins. It is expressed on erythrocytes and erythroblasts, and serves as a receptor for LFA-1, Mac-1, and CD11c/CD18, plus alpha4 beta1 and alpha-V containing integrins. ICAM-4 is suggested to bind to Mac-1 on macrophages, allowing for its phagocytosis in senescence. Mature human ICAM-4 is a 249 amino acid (aa) type I transmembrane glycoprotein. It possesses a 218 aa extracellular region (aa 23-240) that contains two C2-type Ig-like domains (aa 62-124 and 146-217), and a 10 aa C-terminal cytoplasmic tail. ICAM-4 may form 85 kDa homodimers. There are three potential isoform variants. One shows a five aa substitution for aa 233-271, a second contains a 15 aa substitution for aa 14-29, and a third possesses a 141 aa substitution for aa 132-271. Over aa 31-240, human ICAM-4 shares 71% aa identity with mouse ICAM-4.
Long Name
Intercellular Adhesion Molecule 4
Alternate Names
CD242, ICAM4
Gene Symbol
ICAM4
UniProt
Additional ICAM-4 Products
Product Documents for Human ICAM-4 Antibody
Product Specific Notices for Human ICAM-4 Antibody
For research use only
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