Human IFN-gamma Fluorescein-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC285F
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 25723
Product Specifications
Immunogen
E. coli-derived recombinant human IFN-gamma
Gln24-Gln166
Accession # AAP20098.1
Gln24-Gln166
Accession # AAP20098.1
Specificity
Detects human IFN-gamma in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IFN-gamma, recombinant rat IFN-gamma, or recombinant porcine IFN-gamma is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human IFN-gamma Fluorescein-conjugated Antibody
Detection of IFN‑ gamma in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) treated with 50 ng/mL PMA, 1 µg/mL Ionomycin, and 3 µM Monensin overnight were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human IFN-gamma Fluorescein-conjugated Monoclonal Antibody (Catalog # IC285F) or (B) Mouse IgG2BFluorescein Isotype Control (Catalog # IC0041F). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications for Human IFN-gamma Fluorescein-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PMA, ionomycin, and monensin were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PMA, ionomycin, and monensin were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using IC285F in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IFN-gamma
Long Name
Interferon gamma
Alternate Names
IFG, IFI, IFNG, IFNgamma
Entrez Gene IDs
Gene Symbol
IFNG
UniProt
Additional IFN-gamma Products
Product Documents for Human IFN-gamma Fluorescein-conjugated Antibody
Product Specific Notices for Human IFN-gamma Fluorescein-conjugated Antibody
For research use only
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