Detects human IL-1 RII in direct ELSAs and Western blots. When used in combination with the biotinylated human IL-1 RII affinity purified polyclonal detection antibody (Catalog # BAF263) in sandwich ELISAs, no significant cross-reactivity or interference was observed with recombinant human (rh) IL-1ra, rhIL-1 RI, recombinant mouse IL-1ra, or recombinant rat IL-1ra.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human IL-1 RII Alexa Fluor® 700-conjugated Antibody

Detection of IL‑1 RII in Human PBMCs stimulated to induce Tregs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs), stimulated to induce Regulatory T Cells (Tregs) and gated on CD4⁺, were treated with 10 ug/mL Anti-CD3, 5 ug/mL Anti-CD28, 10 ng/mL Recombinant Human TGF-beta 1 (240-B), and 20 ng/mL Recombinant Human IL-2 (202-IL) for 48 hours and stained with Rat Anti-Human LRRC32/GARP PE-conjugated Monoclonal Antibody and either (A) Mouse Anti-Human IL-1 RII Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB663N) or (B) Mouse IgG₁Alexa Fluor 700 Isotype Control (IC002N). View our protocol for Staining Membrane-associated Proteins.

Detection of IL-1 RII in HDLM-2 cells by Flow Cytometry

HDLM-2 cells were stained with Mouse Anti-Human IL-1 RII Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB663N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IL-1 RII Alexa Fluor® 700-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

5 µL/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs), stimulated to induce Regulatory T Cells (Tregs) and gated on CD4⁺, were treated with Anti-CD3, Anti-CD28, Recombinant Human TGF‑ beta1 (Catalog # 240-B), and Recombinant Human IL‑2 (Catalog # 202-IL)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-1 RII

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes, and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex. An IL-1 receptor accessory protein that can heterodimerize with the type I receptor in the presence of IL-1 alpha or IL-1 beta, but not IL-1ra, was identified (1). This type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling type I receptor complex. Recombinant IL-1 soluble receptor type II is a potent antagonist of IL-1 action.

References

Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.

Long Name

Interleukin 1 Receptor II

Alternate Names

CD121b, IL-1 R beta, IL-1RII, IL1R2, IL1RII

Entrez Gene IDs

7850 (Human); 16178 (Mouse)

Gene Symbol

IL1R2

UniProt

P27930

Additional IL-1 RII Products

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SDS

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Product Specific Notices for Human IL-1 RII Alexa Fluor® 700-conjugated Antibody

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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Human IL-1 RII Alexa Fluor® 700-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB663N

Key Product Details

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Product Specifications

Immunogen

Specificity