Human IL-10 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB217
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Neutralization, Simple Western, Western Blot
Cited:
Affinity Chromatography, Blocking, ELISA Development (Capture), Flow Cytometry, Luminex Development, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 23738
Product Specifications
Immunogen
E. coli-derived recombinant human IL-10
Ser19-Asn178
Accession # P22301
Ser19-Asn178
Accession # P22301
Specificity
Detects human IL-10 in Western blots. In Western blots, this antibody shows approximately 5% cross-reactivity with recombinant mouseIL-10.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IL-10 Antibody
Detection of Human IL‑10 by Western Blot.
Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB217) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human IL‑10 by Simple WesternTM.
Simple Western lane view shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10, loaded at 0.2 mg/mL. A specific band was detected for IL‑10 at approximately 23 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB217) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Cell Proliferation Induced by IL‑10 and Neutralization by Human IL-10 Antibody.
Recombinant Human IL-10 (Catalog # 217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB217). At 2 µg/mL, this antibody will neutralize >60% Recombinant Human IL-10 induced bioactivity.Applications for Human IL-10 Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human IL-10
Sample: HEK293 human embryonic kidney cell line transfected with human IL-10
Western Blot
2 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human IL-10
Sample: HEK293 human embryonic kidney cell line transfected with human IL-10
Neutralization
Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. At 2 µg/mL, this antibody will neutralize >60% of the bioactivity due to 5 ng/mL Recombinant Human IL‑10.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-10
Long Name
Interleukin 10
Alternate Names
CSIF, GVHDS, IL10, IL10A, TGIF
Entrez Gene IDs
Gene Symbol
IL10
UniProt
Additional IL-10 Products
Product Documents for Human IL-10 Antibody
Product Specific Notices for Human IL-10 Antibody
For research use only
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