Human IL-18/IL-1F4 Antibody

R&D Systems, part of Bio-Techne | Catalog # D045-3

R&D Systems, part of Bio-Techne
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D045-3
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D045-6
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation

Cited:

Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 159-12B

View all IL-18/IL-1F4 Antibodies »

Product Specifications

Immunogen

Human IL-18/IL-1F4

Specificity

This antibody reacts with human IL-18 on Immunoprecipitation.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human IL-18/IL-1F4 Antibody

Application
Recommended Usage

Immunoprecipitation

5 μg/ 0.5 μg recombinant human IL-18

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

100 μg IgG in 100 μL PBS containing 50% glycerol, pH 7.2. Contains no preservatives.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody solution is stable for one year from the date of purchase when stored at -20°C.

Background: IL-18/IL-1F4

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.

References

  1. Nussbaumer, O., et al., Blood 118, 2743-2751 (2011)
  2. Tu, A., et al., J. Exp. Med. 205, 233-244 (2008)
  3. Wu, C., J. Immunol. 170, 5571-5577 (2003)
  4. Sugawara, S., J. Immunol. 167, 6568-6575 (2001)
  5. Dao, T., et al., Cell Immunol. 173, 230-235 (1996)
  6. Micallef, M., et al., Eur. J. Immunol. 26, 1647-1651 (1996)
  7. Ushio, S., et al., J. Immunol. 156, 4274-4279 (1996)
  8. Okamura, H., et al., Nature 378, 88-91 (1995)

Long Name

Interleukin 18

Alternate Names

IGIF, IL-1F4, IL-1g, IL18

Entrez Gene IDs

3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)

Gene Symbol

IL18

Additional IL-18/IL-1F4 Products

Product Documents for Human IL-18/IL-1F4 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL-18/IL-1F4 Antibody

For research use only

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