Antibody Array Development, ELISA Detection, ELISA Development (Capture), ELISA Development (Detection), ELISA Microarray Development, Luminex Development

Label

Biotin

Antibody Source

Monoclonal Rat IgG_2A Clone # 159-12B

View all IL-18/IL-1F4 Antibodies »

Product Specifications

Immunogen

Human IL-18/IL-1F4

Specificity

This antibody reacts with human IL-18 on ELISA.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Applications for Human IL-18/IL-1F4 Biotinylated Antibody

Application

Recommended Usage

Human IL-18/IL-1F4 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:

Detection Reagent: Human IL-18/IL-1F4 Antibody (Catalog # D044-3)
Standard: Recombinant Human IL-18/IL-1F4 Protein (Catalog # B001-5)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

25 μg IgG in 50 μL volume of PBS containing 1% BSA and 0.09% sodium azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody solution is stable for one year from the date of purchase when stored at 4°C.

Background: IL-18/IL-1F4

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.

References

McCurnin, D., et al., Pediatrics 121, 945-956 (2008)
Haveman, L. M., et al., Int. Immunol. 18, 1521-1529 (2006)
Shigehara, K., et al., J. Immunol. 166, 642-649 (2001)
Dao, T., et al. Cell Immunol. 173, 230-235 (1996)
Micallef, M., et al. Eur. J. Immunol. 26, 1647-1651 (1996)
Ushio, S., et al. J. Immunol. 156, 4274-4279 (1996)
Okamura, H., et al. Nature 378, 88-91 (1995)

Long Name

Interleukin 18

Alternate Names

IGIF, IL-1F4, IL-1g, IL18

Entrez Gene IDs

3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)

Gene Symbol

IL18

Additional IL-18/IL-1F4 Products

Product Documents for Human IL-18/IL-1F4 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human IL-18/IL-1F4 Biotinylated Antibody

For research use only

Citations
Reviews

Human IL-18/IL-1F4 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # D045-6

Key Product Details

Species Reactivity

Validated:

Cited:

Applications