Human IL-2 Antibody Best Seller
R&D Systems, part of Bio-Techne | Catalog # MAB202
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Immunocytochemistry, Intracellular Staining by Flow Cytometry, Neutralization
Cited:
Bioassay, ELISA Detection, Flow Cytometry, Immunocytochemistry, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5334
Product Specifications
Immunogen
E. coli-derived recombinant human IL-2
Ala21-Thr153
Accession # NP_000577
Ala21-Thr153
Accession # NP_000577
Specificity
Detects human IL-2 in direct ELISAs. Does not cross-react with recombinant IL-2 from mouse, rat, pig, or cotton rat.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IL-2 Antibody
Cell Proliferation Induced by IL-2 and Neutralization by Human IL-2 Antibody.
Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB202). The ND50 is typically 0.015-0.03 µg/mL.
IL‑2 in Human PBMCs.
IL-2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB202) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human IL-2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells treated with calcium ionomycin and PMA
Sample: Immersion fixed human peripheral blood mononuclear cells treated with calcium ionomycin and PMA
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells treated with PMA and Ca2+ ionomycin, fixed with paraformaldehyde, and permeabilized with saponin
Sample: Human peripheral blood mononuclear cells treated with PMA and Ca2+ ionomycin, fixed with paraformaldehyde, and permeabilized with saponin
Neutralization
Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 276. The Neutralization Dose (ND50) is typically 0.015-0.03 µg/mL in the presence of 2 ng/mL Recombinant Human IL‑2.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using MAB202 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-2
Long Name
Interleukin 2
Alternate Names
Aldesleukin, IL2, Proleukin, TCGF
Entrez Gene IDs
Gene Symbol
IL2
UniProt
Additional IL-2 Products
Product Documents for Human IL-2 Antibody
Product Specific Notices for Human IL-2 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...