IL-2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB202R) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Cell Proliferation Induced by IL-2 and Neutralization by Human IL-2 Antibody.

Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB202). The ND₅₀ is typically 0.015-0.03 µg/mL.

Detection of IL-2 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) (A) treated with Cell Activation Cocktail 500x (5476) for 5 hours or (B) resting were stained with Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB202R) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (FAB100P). Quadrant markers were set based on Mouse IgG1 isotype control antibody (MAB002) . To facilitate intracellular staining, cells were fixed and permeabilized using FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.

Applications for Human IL-2 Antibody

Application

Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with Cell Activation Cocktail 500x (Catalog # 5476) for 5 hours, fixed and permeabilized using FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012)

Neutralization

Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 276. The Neutralization Dose (ND₅₀) is typically 0.015-0.03 µg/mL in the presence of 2 ng/mL Recombinant Human IL‑2.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2

Interleukin-2 (IL-2) is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha, beta, and gamma chains.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Gene Symbol

IL2

UniProt

NP_000577

Additional IL-2 Products

Product Documents for Human IL-2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IL-2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human IL-2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB202R

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all IL-2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human IL-2 Antibody

IL-2 in Human PBMCs.