Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10346
Recombinant Monoclonal Antibody For Research Use Only
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Neutralization
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # Hu137
Product Specifications
Immunogen
Human IL-6R alpha
Specificity
Detects Human IL-6R alpha based on Sarilumab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Sarilumab. This product is for research use only.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Antibody
Detection of IL-6R alpha in HEK293 Human cell line Transfected with Human IL-6R alpha and eGFP by Flow Cytometry.
HEK293 Human cell line transfected with (A) Human IL-6R alpha or (B) irrelevant protein, and eGFP was stained with Human Anti-Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Monoclonal Antibody (Catalog # MAB10346) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.
Detection of IL-6R alpha in U937 human histiocytic lymphoma cell line by Flow Cytometry.
U937 human histiocytic lymphoma cell line was stained with Human Anti-Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Monoclonal Antibody (Catalog # MAB10346, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.
IL-6 R alpha Enhancement of IL-6-dependent Inhibition of Cell Proliferation and Neutralization by Human IL-6 R alpha Antibody.
Recombinant Human IL-6 Ra (Catalog # 227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant Human IL-6 Ra (100 ng/mL) is neutralized (green line) by increasing concentrations of Human Anti-Human IL-6 Ra (Research Grade Sarilumab) Monoclonal Antibody (Catalog # MAB10346). The ND50 is typically 0.04-0.2 µg/mL.Applications for Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human cell line Transfected with Human IL-6R alpha and eGFP and U937 human histiocytic lymphoma cell line
Sample: HEK293 Human cell line Transfected with Human IL-6R alpha and eGFP and U937 human histiocytic lymphoma cell line
Neutralization
Measured by its ability to neutralize IL‑6 R alpha-mediated enhancement of IL‑6-dependent Inhibition of cell proliferation in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND50) is typically 0.04-0.2 µg/mL in the presence of 100 ng/mL Recombinant Human IL‑6 R alpha and 30 ng/mL Recombinant Human IL‑6.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-6R alpha
Long Name
Interleukin 6 Receptor alpha
Alternate Names
CD126, IL-6 R alpha, IL-6Ra, IL6R, IL6R alpha
Gene Symbol
IL6R
Additional IL-6R alpha Products
Product Documents for Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Antibody
Product Specific Notices for Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Antibody
For research use only
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