Human IL-6R alpha (Research Grade Tocilizumab Biosimilar) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11482
Recombinant Monoclonal Antibody For Research Use Only
Key Product Details
Species Reactivity
Human
Applications
Blockade of Receptor-ligand Interaction, ELISA, Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # Hu203
Product Specifications
Immunogen
E. coli-derived recombinant human IL-6R alpha
Arg387-Arg468
Accession # P08887
Arg387-Arg468
Accession # P08887
Specificity
Detects human IL-6R alpha in direct ELISAs.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Scientific Data Images for Human IL-6R alpha (Research Grade Tocilizumab Biosimilar) Antibody
Detection of IL-6R alpha in HEK293 cells transfected with hIL-6Ra and eGFP by Flow Cytometry
HEK293 cells transfected with hIL-6Ra and eGFP were stained with Human Anti-Human IL-6R alpha (Research Grade Tocilizumab Biosimilar) Monoclonal Antibody (Catalog # MAB11482, filled histogram) followed by Allophycocyanin-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135) or unstained cells (open blue histogram). View our protocol for Staining Membrane-associated Proteins.
IL-6 binding to IL-6Ra transfected HEK293 Human Cell line is Blocked by Human IL-6Ra (Tocilizumab) Antibody.
In a functional flow cytometry test, biotinylated recombinant IL-6 (50ng/mL, BT7270B) binds to HEK293 human embyonic kidney cell line transfected with human IL-6Ra and eGFP. (A) Binding is blocked by 25 ug/mL of Human anti-Human IL-6Ra Monoclonal Antibody (Catalog# MAB11482) or (B) Protein binding only. Protein binding was detected by staining cells with Streptavidin-APC (F0050)
Human IL-6R alpha ELISA Standard Curve
Direct ELISA binding curve demonstrating the recognition of Human Anti-Human IL-6R alpha (Research Grade Tocilizumab Biosimilar) Monoclonal Antibody (Catalog # MAB11482) to IL-6R alpha. The target protein was coated onto the microplate well surface, followed by binding of the antibody. A goat anti-human HRP conjugate was used for detection.Applications for Human IL-6R alpha (Research Grade Tocilizumab Biosimilar) Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
0.25 µg/106 cells
Sample: Blocking assay in hIL-6Ra HEK/eGFP transfecants (see details below)
Sample: Blocking assay in hIL-6Ra HEK/eGFP transfecants (see details below)
ELISA
This antibody functions as an ELISA detection antibody for the specific antigen in direct ELISA. Colorimetric detection is performed after addition of a suitable substrate.
Flow Cytometry
0.25 µg/106 cells
Sample: hIL-6Ra HEK/eGFP transfectants
Sample: hIL-6Ra HEK/eGFP transfectants
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-6R alpha
The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex (1‑4). Within the extracellular domain, human IL‑6 R alpha shares 52% aa sequence identity with
mouse and rat IL‑6 R alpha.
A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alphas have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL‑6/IL‑6 R alpha complex to membrane-bound gp130 (6, 7).
References
- Yamasaki et al. (1988) Science 241:825.
- Baumann et al. (1990) J. Biol. Chem. 265:19853.
- Hibi et al. (1990) Cell 63:1149.
- Schooltink et al. (1991) Eur. J. Biochem. 277:659.
- Novick et al. (1989) J. Exp. Med. 170:1409.
- Yasukawa et al. (1990) J. Biochem. 108:673.
- Saito et al. (1991) J. Immunology 147:168.
Long Name
Interleukin 6 Receptor alpha
Alternate Names
CD126, IL-6 R alpha, IL-6Ra, IL6R, IL6R alpha
Gene Symbol
IL6R
UniProt
Additional IL-6R alpha Products
Product Documents for Human IL-6R alpha (Research Grade Tocilizumab Biosimilar) Antibody
Product Specific Notices for Human IL-6R alpha (Research Grade Tocilizumab Biosimilar) Antibody
For research use only
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