Human IMPA1/IMP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9890
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 984604
Product Summary for Human IMPA1/IMP1 Antibody
Immunogen
Synthetic peptide containing human IMPA/IMP1 Peptide
Accession # P29218
Accession # P29218
Specificity
Detects human IMPA1/IMP1 Peptide in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IMPA1/IMP1 Antibody
IMPA1/IMP1 in HCT‑116 Human Cell Line.
IMPA1/IMP1 was detected in immersion fixed HCT-116 human colorectal carcinoma cell line using Mouse Anti-Human IMPA1/IMP1 Monoclonal Antibody (Catalog # MAB9890) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
IMPA1/IMP1 in Human Brain.
IMPA1/IMP1 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human IMPA1/IMP1 Monoclonal Antibody (Catalog # MAB9890) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human IMPA1/IMP1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HCT-116 human colorectal carcinoma cell line
Sample: Immersion fixed HCT-116 human colorectal carcinoma cell line
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain
Sample: Immersion fixed paraffin-embedded sections of human brain
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB9890 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IMPA1/IMP1
IMPA-1/inositol(myo)-1(or 4)-monophosphatase 1 is a ubiquitous enzyme with broad specificity, which is responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides. IMPA-1 dephosphorylates myo-inositol monophosphatate to modulate intracellular signal transduction. IMPA-1 forms homodimers, and is abundantly expressed in brain. The magnesium-dependent phosphatase activity is inhibited by lithium, thereby depressing myo-inositol production, which may explain the anti-depressive and anti-manic effects of lithium therapy in bipolar disorder.
Long Name
Inositol Monophosphatase 1
Alternate Names
IMP, IMP 1, IMP1, IMPA, IMPase 1, MRT59, Myo-Inositol Monophosphatase 1
Gene Symbol
IMPA1
UniProt
Additional IMPA1/IMP1 Products
Product Documents for Human IMPA1/IMP1 Antibody
Product Specific Notices for Human IMPA1/IMP1 Antibody
For research use only
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