Detects human Integrin beta7 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta1, beta2, beta3, beta4, beta5, beta6, beta8, recombinant mouse Integrin beta4, or beta7 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Integrin beta7 Antibody

Detection of Integrin beta7 in Human Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin beta7 Monoclonal Antibody (Catalog # MAB4669, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Integrin beta7 in Human PBMCs.

Integrin beta7 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human Integrin beta7 Monoclonal Antibody (Catalog # MAB4669) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Integrin beta7 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood lymphocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 7

Human Integrin beta7 (also Integrin betaP and M290 IEL Ag) is a 120 kDa member of the Integrin beta chain family of molecules. It is a type I transmembrane glycoprotein that contains a 704 amino acid (aa) extracellular domain (ECD) (aa 20‑723) and a 52 aa cytoplasmic tail. The ECD contains one von Willebrand factor A domain (aa 150‑389) and four cysteine‑rich EGF‑like repeats. One splice variant exists that shows a deletion of aa 501‑648. Integrin beta7 forms a nondisulfide linked heterodimer with alpha4 and alphaE integrins. alpha4 beta7 binds fibronectin, VCAM-1 and MADCAM1, while alphaE beta7 binds E-cadherin. The ECD of human beta7 is 87% aa identical to the ECD of mouse beta7.

Alternate Names

ITGB7

Entrez Gene IDs

3695 (Human); 16421 (Mouse); 25713 (Rat); 102137082 (Cynomolgus Monkey)

Gene Symbol

ITGB7

UniProt

P26010

Additional Integrin beta 7 Products

Product Documents for Human Integrin beta7 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Integrin beta7 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Integrin beta7 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4669

Key Product Details

Species Reactivity

Validated:

Cited:

Applications