BaF3 mouse pro-B cell line transfected with human KIRDL3/CD158z was stained with Rabbit Anti-Human KIR3DL3/CD158z Monoclonal Antibody (Catalog # MAB8919, filled histogram) or Rabbit Anti-Human KIR2DS5/CD158g Monoclonal Antibody (Catalog # MAB8920, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).

Applications for Human KIR3DL3/CD158z Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: BaF3 mouse pro-B cell line transfected with human KIRDL3/CD158z

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KIR3DL3/CD158z

KIR3DL3 is a member of the killer-immunoglobulin-like receptors (KIR), a functionally diverse family of transmembrane receptors expressed primarily on NK cells. KIR3DL3 is a “framework” KIR gene, and is present in all individuals, in contrast to non-framework KIRs, whose gene content varies by KIR haplotype. KIR3DL3 is expressed at low or undetectable levels in peripheral blood NK cells, but expression can be induced in NK cell lines by demethylation agents, indicating that promoter DNA methylation inhibits KIR3DL3 transcription. Despite the lack of evidence for surface protein expression, KIR3DL3 mRNA expression has been detected in both decidual and peripheral blood NK cells in normal, disease-free conditions.

Long Name

Killer Cell Immunoglobulin-like Receptor, Three Domain Long Cytoplasmic Tail, 3

Alternate Names

CD158z, KIR3DL7, KIR44, KIRC1

Entrez Gene IDs

115653 (Human)

Gene Symbol

KIR3DL3

UniProt

Q8N743

Additional KIR3DL3/CD158z Products

Product Documents for Human KIR3DL3/CD158z Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human KIR3DL3/CD158z Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human KIR3DL3/CD158z Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8919

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all KIR3DL3/CD158z Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human KIR3DL3/CD158z Antibody

Detection of KIR3DL3/CD158z in BaF3 Mouse Cell Line Transfected with Human KIRDL3/CD158z by Flow Cytometry.