BaF3 mouse pro-B cell line transfected with human KIR2DS5/CD158g was stained with Rabbit Anti-Human KIR2DS5/CD158g Monoclonal Antibody (Catalog # MAB8920, filled histogram) or Rabbit Anti-Human KIR3DL3/CD158z Monoclonal Antibody (Catalog # MAB8919, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).

Detection of KIR2DS5/CD158g in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human KIR2DS5/CD158g Monoclonal Antibody (Catalog # MAB8920) or (B) Rabbit IgG Isotype Control (Catalog # MAB1050) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human KIR2DS5/CD158g Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: BaF3 mouse pro-B cell line transfected with human KIR2DS5/CD158g and Human Peripheral Blood Mononuclear Cells (PBMCs)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KIR2DS5/CD158g

Killer-immunoglobulin-like receptors (KIR) are polymorphous activating and inhibitory receptors expressed on the surface of NK cells and some T cells. KIR genes are highly homologous. KIR proteins expressing the long (L) cytoplasmic domain are inhibitory, while KIRs with short (S) cytoplasmic domains are activating. Thus, KIR2DS5 is an activating receptor for NK cells, and is thought to play a role in NK cell function in response to a number of conditions. While many KIR proteins bind HLA-class I molecules, the ligand(s) for KIR2DS5 remain unknown.

Long Name

Killer Cell Immunoglobulin-like Receptor, Two Domains, Short Cytoplasmic Tail, 5

Alternate Names

CD158g, NKAT9

Entrez Gene IDs

3810 (Human)

Gene Symbol

KIR2DS5

UniProt

Q14953

Additional KIR2DS5/CD158g Products

Product Documents for Human KIR2DS5/CD158g Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human KIR2DS5/CD158g Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human KIR2DS5/CD158g Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8920

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all KIR2DS5/CD158g Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human KIR2DS5/CD158g Antibody

Detection of KIR2DS5/CD158g in BaF3 Mouse Cell Line Transfected with Human KIR2DS5/CD158g by Flow Cytometry.