Human Laminin alpha 2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2143
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 1003531
Product Specifications
Immunogen
Chinese Hamster Ovary cell line, CHO-derived human Laminin alpha 2
Lys2145-Asn3122
Accession # P24043
Lys2145-Asn3122
Accession # P24043
Specificity
Detects human Laminin alpha 2 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Laminin alpha 2 Antibody
Laminin alpha 2 in Human Heart.
Laminin alpha 2 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Laminin alpha 2 Monoclonal Antibody (Catalog # MAB2143) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Laminin alpha 2 in U2OS and MCF-7 Human Cell Lines.
Laminin alpha 2 was detected in immersion fixed U2OS human osteosarcoma cell line (positive stain) and MCF-7 human breast cancer cell line (negative stain) using Mouse Anti-Human Laminin alpha 2 Monoclonal Antibody (Catalog # MAB2143) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Laminin alpha 2 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed U2OS human osteosarcoma cell line and MCF-7 human breast cancer cell line
Sample: Immersion fixed U2OS human osteosarcoma cell line and MCF-7 human breast cancer cell line
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart
Sample: Immersion fixed paraffin-embedded sections of human heart
Reviewed Applications
Read 1 review rated 5 using MAB2143 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Laminin alpha 2
Alternate Names
LAMA2, LAMM, Merosin
Gene Symbol
LAMA2
UniProt
Additional Laminin alpha 2 Products
Product Documents for Human Laminin alpha 2 Antibody
Product Specific Notices for Human Laminin alpha 2 Antibody
For research use only
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