Detects human Laminin alpha3/Laminin‑5 in direct ELISAs and Western blots. In direct ELISAs, no cross reactivity with recombinant human (rh) Laminin alpha1, beta1, gamma2, or recombinant mouse Laminin alpha4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Laminin alpha3/Laminin-5 Antibody

Detection of Human Laminin alpha3/Laminin‑5 by Western Blot.

Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Laminin a3/ Laminin-5 Monoclonal Antibody (Catalog # MAB21441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Laminin a3/Laminin-5 at approximately 190 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Laminin alpha3/Laminin‑5 in NHEK Human Cell Line.

Laminin a3/Laminin-5 was detected in immersion fixed NHEK human normal epidermal keratinocytes treated with 10ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B) for 24 hours using Mouse Anti-Human Laminin a3/Laminin-5 Monoclonal Antibody (Catalog # MAB21441) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the perinuclear space. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Laminin alpha3/Laminin‑5 in Human Stomach.

Laminin a3/Laminin-5 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human Laminin a3/Laminin-5 Monoclonal Antibody (Catalog # MAB21441) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of epithelial cells in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Applications for Human Laminin alpha3/Laminin-5 Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NHEK human normal epidermal keratinocytes treated with Recombinant Human TGF‑ beta1 (Catalog # 240-B)

Immunohistochemistry

5 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Laminin alpha3, see our available Western blot detection antibodies

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: U2OS Human cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Western Blot

2 µg/mL
Sample: Human lung tissue under non-reducing conditions only

Reviewed Applications

Read 3 reviews rated 5 using MAB21441 in the following applications:

Immunofluorescence (1 Review)
Immunohistochemistry (1 Review)
Immunohistochemistry frozen (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Laminin alpha 3/Laminin-5

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. Through interactions with integrins, dystroglycan and other receptors, laminins contribute to cell differentiation, cell shape and migration, and maintenance of tissue phenotypes and survival. Laminin alpha3/Laminin-5, also known as epiligrin, includes alpha3, beta3, and gamma2 subunits. It is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa and other epithelial glands and contributes to initiation and maintenance of epithelial cell anchorage to the underlying connective tissue. Within aa 21‑1713 of the alpha3 subunit, human and mouse share 77% amino acid sequence identity.

Alternate Names

Epiligrin, LAMA3, Laminin-5, Laminin5

Entrez Gene IDs

3909 (Human)

Gene Symbol

LAMA3

UniProt

NP_000218

Additional Laminin alpha 3/Laminin-5 Products

Product Documents for Human Laminin alpha3/Laminin-5 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Laminin alpha3/Laminin-5 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Laminin alpha3/Laminin-5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB21441

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications