HEK293 human embryonic kidney cell line transfected with human Lgr6 and eGFP was stained with either (A) Mouse Anti-Human Lgr6 Monoclonal Antibody (Catalog # MAB84581) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Lgr6 in HEK293 Human Cell Line.

Lgr6 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with Lgr6 using Mouse Anti-Human Lgr6 Monoclonal Antibody (Catalog # MAB84581) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

R-Spondin 4 Binding to Lgr6-transfected HEK293 Human Cell Line is Blocked by Human Lgr6 Antibody.

In a functional flow cytometry test, biotinylated recombinant human R-Spondin 4 (100 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with human Lgr6 (dark orange filled histogram). Binding is completely blocked (light orange filled histogram) by 1 µg/mL of Mouse Anti-Human Lgr6 Monoclonal Antibody (Catalog # MAB84581). Mouse IgG2A Isotype Control (Catalog # MAB003) at 1 µg/mL was used as a control (blue line). Cells were stained with Streptavidin-APC (Catalog # F0050).

Applications for Human Lgr6 Antibody

Application

Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional flow cytometry test, biotinylated recombinant human R-Spondin 4 (100 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with human Lgr6. Binding is completely blocked by 1 μg/mL of Mouse Anti-Human Lgr6 Monoclonal Antibody (Catalog # MAB84581).

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human Lgr6 and eGFP

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line transfected with Lgr6

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lgr6

Lgr6 is a glycoprotein hormone receptor phosphorylated upon DNA damage, probably by ATM or ATR. It is a member of the leucine-rich repeat-containing subgroup of the G protein-coupled 7-transmembrane protein superfamily. Lgr6 is a high affinity receptor for R-Spondins 1-3 and potentially functions as a tumor suppressor despite its positive effect on Wnt/ beta-Catenin signaling.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 6

Alternate Names

FLJ14471, gonadotropin receptor, GPCR, leucine-rich repeat containing G protein-coupled receptor 6, leucine-rich repeat-containing G protein-coupled receptor 6, leucine-rich repeat-containing G-protein coupled receptor 6, VTS20631

Entrez Gene IDs

59352 (Human); 329252 (Mouse); 498233 (Rat)

Gene Symbol

LGR6

UniProt

Q9HBX8

Additional Lgr6 Products

Product Documents for Human Lgr6 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Lgr6 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Lgr6 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB84581

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all Lgr6 Antibodies »

Product Summary for Human Lgr6 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Lgr6 Antibody

Detection of Lgr6 in HEK293 Human Cell Line Transfected with Human Lgr6 and eGFP by Flow Cytometry.